Gelsolin/GSN Antibody (20) - BSA Free
Novus Biologicals | Catalog # NBP1-05161
![Western Blot: Gelsolin/GSN Antibody (20)BSA Free [NBP1-05161]](https://resources.rndsystems.com/images/products/Gelsolin-GSN-Antibody-20-BSA-Free-Western-Blot-NBP1-05161-img0002.jpg "Western Blot: Gelsolin/GSN Antibody (20)BSA Free [NBP1-05161]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 20
Format
BSA Free
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Product Specifications
Immunogen
Gelsolin purified from human plasma
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Concentration for any given batch will be between 0.85 - 1.15 mg/mL.
Scientific Data Images for Gelsolin/GSN Antibody (20) - BSA Free
Western Blot: Gelsolin/GSN Antibody (20)BSA Free [NBP1-05161]
Western Blot: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil![Immunocytochemistry/ Immunofluorescence: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161]](https://resources.rndsystems.com/images/products/Gelsolin-GSN-Antibody-20-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05161-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161]")
Immunocytochemistry/ Immunofluorescence: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161]
Immunocytochemistry/Immunofluorescence: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161] - Human cell line U-251 MG shows positivity in actin filaments. Antibody dilution: 1:2000![Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161]](https://resources.rndsystems.com/images/products/Gelsolin-GSN-Antibody-20-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05161-img0003.jpg "Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161]")
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161]
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161] - Human Kidney.Applications for Gelsolin/GSN Antibody (20) - BSA Free
Application
Recommended Usage
ELISA
1:4000
Immunocytochemistry/ Immunofluorescence
1:2000
Immunohistochemistry
1:6000
Immunohistochemistry-Paraffin
1:6000
Western Blot
1:100-1:2000
Application Notes
ELISA: NBP1-05161 does not work as a capture antibody in sandwich ELISA.
WB: NBP1-05161 can be used in Western blotting.
IHC: NBP1-05161 can be used in Immunohistochemistry.
IF: NBP1-05161 can be used in Immunofluorescence.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM Phosphate (pH 7.4) and 0.5M NaCl
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Gelsolin/GSN
Alternate Names
ADF, AGEL, Brevin, GSN
Gene Symbol
GSN
Additional Gelsolin/GSN Products
Product Documents for Gelsolin/GSN Antibody (20) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Gelsolin/GSN Antibody (20) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Gelsolin/GSN Antibody (20) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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