Gelsolin/GSN Antibody (20) - BSA Free
Novus Biologicals | Catalog # NBP1-05161
![Western Blot: Gelsolin/GSN Antibody (20)BSA Free [NBP1-05161]](https://resources.rndsystems.com/images/products/Gelsolin-GSN-Antibody-20-BSA-Free-Western-Blot-NBP1-05161-img0002.jpg "Western Blot: Gelsolin/GSN Antibody (20)BSA Free [NBP1-05161]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 20
Format
BSA Free
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Product Specifications
Immunogen
Gelsolin purified from human plasma
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Gelsolin/GSN Antibody (20) - BSA Free
Western Blot: Gelsolin/GSN Antibody (20)BSA Free [NBP1-05161]
Western Blot: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil![Immunocytochemistry/ Immunofluorescence: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161]](https://resources.rndsystems.com/images/products/Gelsolin-GSN-Antibody-20-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05161-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161]")
Immunocytochemistry/ Immunofluorescence: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161]
Immunocytochemistry/Immunofluorescence: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161] - Human cell line U-251 MG shows positivity in actin filaments. Antibody dilution: 1:2000![Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161]](https://resources.rndsystems.com/images/products/Gelsolin-GSN-Antibody-20-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05161-img0003.jpg "Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161]")
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161]
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161] - Human Kidney.Applications for Gelsolin/GSN Antibody (20) - BSA Free
Application
Recommended Usage
ELISA
1:4000
Immunocytochemistry/ Immunofluorescence
1:2000
Immunohistochemistry
1:6000
Immunohistochemistry-Paraffin
1:6000
Western Blot
1:100-1:2000
Application Notes
ELISA: NBP1-05161 does not work as a capture antibody in sandwich ELISA.
WB: NBP1-05161 can be used in Western blotting.
IHC: NBP1-05161 can be used in Immunohistochemistry.
IF: NBP1-05161 can be used in Immunofluorescence.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM Phosphate (pH 7.4) and 0.5M NaCl
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Gelsolin/GSN
Alternate Names
ADF, AGEL, Brevin, GSN
Gene Symbol
GSN
Additional Gelsolin/GSN Products
Product Documents for Gelsolin/GSN Antibody (20) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Gelsolin/GSN Antibody (20) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Gelsolin/GSN Antibody (20) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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