Gelsolin/GSN Antibody (GS-2C4)

Novus Biologicals | Catalog # NB110-10067

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Porcine, Bovine, Rabbit, Rat (Negative)

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoaffinity Purification, Microarray

Cited:

Immunohistochemistry-Paraffin, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GS-2C4
View all Gelsolin/GSN Primary Antibodies »

Product Specifications

Immunogen

Full length native protein (purified) (Human).

Epitope

The antibody is directed against an epitope located on the 47 kD peptide derived from a chymotryptic cleavage of gelsolin. This peptide contains the carboxy terminal actin binding site for gelsolin.

Reactivity Notes

Cross-reacts with Human, Cow, Pig and Rabbit. Does not react with Rat.

Localization

Cytoplasmic and Secreted

Specificity

The antibody is specific for an epitope located on the 47 kDa peptide derived from a chymotryptic cleavage of gelsolin. The antibody reacts with plasma and cytoplasmic gelsolin

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Gelsolin/GSN Antibody (GS-2C4)

Western Blot: Gelsolin/GSN Antibody (GS-2C4) [NB110-10067]

Western Blot: Gelsolin/GSN Antibody (GS-2C4) [NB110-10067]

Western Blot: Gelsolin/GSN Antibody (GS-2C4) [NB110-10067] - Human plasma, barium citrate ppt. was separated on SDS-PAGE and probed with Monoclonal AB To Gelsolin (MO) CL:GS-2C4. The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBTBCIP substrate. Antibody dilution 1:2000.

Applications for Gelsolin/GSN Antibody (GS-2C4)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:1000
Application Notes
An additional application for this antibody is immunoaffinity chromatography, for purification of gelsolin, and for detection of gelsolin-actin complexes in cell extracts. WB: Predicted molecular weight: 86 kDa. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 27121327).

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Gelsolin/GSN

Gelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).

Alternate Names

ADF, AGEL, Brevin, GSN

Entrez Gene IDs

2934 (Human)

Gene Symbol

GSN

Additional Gelsolin/GSN Products

Product Documents for Gelsolin/GSN Antibody (GS-2C4)

Certificate of Analysis

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Product Specific Notices for Gelsolin/GSN Antibody (GS-2C4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Citations for Gelsolin/GSN Antibody (GS-2C4)

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Protocols

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