Gelsolin/GSN Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-33604

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (95%), Sheep (96%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Gelsolin/GSN Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Gelsolin/GSN. The exact sequence is proprietary.

Reactivity Notes

Chicken (80%).

Localization

Isoform 2: Cytoplasm, cytoskeleton, Isoform 1: Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Knockout (KO) Validated Rabbit Gelsolin/GSN Antibody - Azide and BSA Free (NBP1-33604) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Gelsolin/GSN Antibody - Azide and BSA Free

Western Blot: Gelsolin/GSN Antibody [NBP1-33604]

Western Blot: Gelsolin/GSN Antibody [NBP1-33604]

Western Blot: Gelsolin/GSN Antibody [NBP1-33604] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Gelsolin/GSN Antibody [NBP1-33604]

Immunocytochemistry/ Immunofluorescence: Gelsolin/GSN Antibody [NBP1-33604]

Immunocytochemistry/Immunofluorescence: Gelsolin/GSN Antibody [NBP1-33604] - Paraformaldehyde-fixed HeLa, using Gelsolin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody [NBP1-33604]

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody [NBP1-33604]

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody [NBP1-33604] - Mouse urinary bladder dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min
Western Blot: Gelsolin/GSN Antibody [NBP1-33604]

Western Blot: Gelsolin/GSN Antibody [NBP1-33604]

Western Blot: Gelsolin/GSN Antibody [NBP1-33604] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Gelsolin/GSN Antibody [NBP1-33604]

Western Blot: Gelsolin/GSN Antibody [NBP1-33604]

Western Blot: Gelsolin/GSN Antibody [NBP1-33604] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Gelsolin/GSN Antibody [NBP1-33604]

Western Blot: Gelsolin/GSN Antibody [NBP1-33604]

Western Blot: Gelsolin/GSN Antibody [NBP1-33604] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Gelsolin/GSN Antibody [NBP1-33604]

Western Blot: Gelsolin/GSN Antibody [NBP1-33604]

Western Blot: Gelsolin/GSN Antibody [NBP1-33604] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody [NBP1-33604]

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody [NBP1-33604]

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody [NBP1-33604] - Mouse brain. Gelsolin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Applications for Gelsolin/GSN Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
IP-Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Gelsolin/GSN

Gelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).

Alternate Names

ADF, AGEL, Brevin, GSN

Gene Symbol

GSN

Additional Gelsolin/GSN Products

Product Documents for Gelsolin/GSN Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Gelsolin/GSN Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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FAQs

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