Gemin 3 Antibody (7B2B1) - BSA Free

Novus Biologicals | Catalog # NBP2-52453

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7B2B1

Format

BSA Free
View all Gemin 3 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Gemin 3 (AA: 725-824) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

92.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Gemin 3 Antibody (7B2B1) - BSA Free

Western Blot: Gemin 3 Antibody (7B2B1)BSA Free [NBP2-52453]

Western Blot: Gemin 3 Antibody (7B2B1)BSA Free [NBP2-52453]

Western Blot: Gemin 3 Antibody (7B2B1) [NBP2-52453] - Analysis using DDX20 mAb against human DDX20 (AA: 725-824) recombinant protein. (Expected MW is 38.4 kDa)
Immunocytochemistry/ Immunofluorescence: Gemin 3 Antibody (7B2B1) - BSA Free [NBP2-52453]

Immunocytochemistry/ Immunofluorescence: Gemin 3 Antibody (7B2B1) - BSA Free [NBP2-52453]

Immunocytochemistry/Immunofluorescence: Gemin 3 Antibody (7B2B1) [NBP2-52453] - Analysis of HeLa cells using DDX20 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Western Blot: Gemin 3 Antibody (7B2B1)BSA Free [NBP2-52453]

Western Blot: Gemin 3 Antibody (7B2B1)BSA Free [NBP2-52453]

Western Blot: Gemin 3 Antibody (7B2B1) [NBP2-52453] - Analysis using DDX20 mAb against HEK293 (1) and DDX20 (AA: 725-824)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Gemin 3 Antibody (7B2B1) - BSA Free [NBP2-52453]

Immunocytochemistry/ Immunofluorescence: Gemin 3 Antibody (7B2B1) - BSA Free [NBP2-52453]

Immunocytochemistry/Immunofluorescence: Gemin 3 Antibody (7B2B1) [NBP2-52453] - Analysis of HeLa cells using DDX20 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
ELISA: Gemin 3 Antibody (7B2B1) - BSA Free [NBP2-52453]

ELISA: Gemin 3 Antibody (7B2B1) - BSA Free [NBP2-52453]

ELISA: Gemin 3 Antibody (7B2B1) [NBP2-52453] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Applications for Gemin 3 Antibody (7B2B1) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Gemin 3

The survival of motor neurons (SMN) gene is the disease gene of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The SMN protein is part of a complex containing several proteins, of which one, SIP1 (SMN interacting protein 1), has been characterized so far. The SMN complex is found in both the cytoplasm and in the nucleus, where it is concentrated in bodies called gems. In the cytoplasm, SMN and SIP1 interact with the Sm core proteins of spliceosomal small nuclear ribonucleoproteins (snRNPs), and they play a critical role in snRNP assembly. In the nucleus, SMN is required for pre-mRNA splicing, likely by serving in the regeneration of snRNPs. A DEAD box putative RNA helicase, named Gemin 3 which is another component of the SMN complex, has been identified. Gemin 3 interacts directly with SMN, as well as with SmB, SmD2 and SmD3. Immunolocalization studies using mAbs to Gemin 3 show that it colocalizes with SMN in gems. Gemin 3 binds SMN via its unique COOH-terminal domain, and SMN mutations found in some SMA patients strongly reduce this interaction. The presence of a DEAD box motif in Gemin 3 suggests that it may provide the catalytic activity that plays a critical role in the function of the SMN complex on RNPs.

Alternate Names

Component of gems 3, DEAD (Asp-Glu-Ala-Asp) box polypeptide 20, DEAD box protein 20, DEAD box protein DP 103, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD, DKFZp434H052, DP103DEAD-box protein DP103, EC 3.6.4.13, Gemin-3, GEMIN3gemin-3, probable ATP-dependent RNA helicase DDX20, SMN-interacting protein

Gene Symbol

DDX20

Additional Gemin 3 Products

Product Documents for Gemin 3 Antibody (7B2B1) - BSA Free

Certificate of Analysis

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Product Specific Notices for Gemin 3 Antibody (7B2B1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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