GFP Antibody (4B10B2) - BSA Free
Novus Biologicals | Catalog # NBP2-22111
Key Product Details
Species Reactivity
Non-species specific
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 4B10B2
Format
BSA Free
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Product Specifications
Immunogen
This GFP antibody was developed against purified recombinant fragment of GFP expressed in E. coli.
Reactivity Notes
Reacts with GFP.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse GFP Antibody (4B10B2) - BSA Free (NBP2-22111) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-GFP Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for GFP Antibody (4B10B2) - BSA Free
![Western Blot: GFP Antibody (4B10B2)BSA Free [NBP2-22111]](https://beta-resources.rndsystems.com/images/products/GFP-Antibody-4B10B2-Western-Blot-NBP2-22111-img0005.jpg "Western Blot: GFP Antibody (4B10B2)BSA Free [NBP2-22111]")
Western Blot: GFP Antibody (4B10B2)BSA Free [NBP2-22111]
Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Expression of GFP in HEK293T cells. This image was submitted via customer Review. Image using the HRP form of this antibody.![Immunocytochemistry/ Immunofluorescence: GFP Antibody (4B10B2) - BSA Free [NBP2-22111]](https://beta-resources.rndsystems.com/images/products/GFP-Antibody-4B10B2-Immunocytochemistry-Immunofluorescence-NBP2-22111-img0004.jpg "Immunocytochemistry/ Immunofluorescence: GFP Antibody (4B10B2) - BSA Free [NBP2-22111]")
Immunocytochemistry/ Immunofluorescence: GFP Antibody (4B10B2) - BSA Free [NBP2-22111]
Immunocytochemistry/Immunofluorescence: GFP Antibody (4B10B2) [NBP2-22111] - Analysis of HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP mouse mAb.![Western Blot: GFP Antibody (4B10B2)BSA Free [NBP2-22111]](https://beta-resources.rndsystems.com/images/products/GFP-Antibody-4B10B2-Western-Blot-NBP2-22111-img0001.jpg "Western Blot: GFP Antibody (4B10B2)BSA Free [NBP2-22111]")
Western Blot: GFP Antibody (4B10B2)BSA Free [NBP2-22111]
Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Analysis using GFP mouse mAb against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4).![Flow Cytometry: GFP Antibody (4B10B2) - BSA Free [NBP2-22111]](https://beta-resources.rndsystems.com/images/products/GFP-Antibody-4B10B2-Flow-Cytometry-NBP2-22111-img0003.jpg "Flow Cytometry: GFP Antibody (4B10B2) - BSA Free [NBP2-22111]")
Flow Cytometry: GFP Antibody (4B10B2) - BSA Free [NBP2-22111]
Flow Cytometry: GFP Antibody (4B10B2) [NBP2-22111] - Analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP mouse mAb.![Western Blot: GFP Antibody (4B10B2)BSA Free [NBP2-22111]](https://beta-resources.rndsystems.com/images/products/GFP-Antibody-4B10B2-Western-Blot-NBP2-22111-img0002.jpg "Western Blot: GFP Antibody (4B10B2)BSA Free [NBP2-22111]")
Western Blot: GFP Antibody (4B10B2)BSA Free [NBP2-22111]
Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Analysis using GFP mouse mAb against extracts from HCC827 cells, untransfected (1) and transfected with GFP (2).Applications for GFP Antibody (4B10B2) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200-1:400
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500-1:2000. Use reported by customer review
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
PBS
Format
BSA Free
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GFP
References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T.,... Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001
2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075
3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S.,... Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9
Long Name
Green Fluorescent Protein
Alternate Names
eGFP, GFPuv
Additional GFP Products
Product Documents for GFP Antibody (4B10B2) - BSA Free
Product Specific Notices for GFP Antibody (4B10B2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for GFP Antibody (4B10B2) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for GFP Antibody (4B10B2) - BSA Free
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Q: We are looking for a rabbit anti-GFP antibody as a primary antibody for immunofluorescence and WB. I've seen that you carry several anti-GFP antibodies and I would like to ask which one you would recommend. The one with Cat.No NB600-308 has been used in a lot of publications and I reckon it is a solid antibody. Is this our best choice, or is there a better alternative?
A: NB600-308 is an excellent GFP antibody and I think it would work very well for you. It has been reviewed and published with which usually makes our customer feel much more confident, seeing as it worked in other people's hand.
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