Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GFR alpha-1/GDNF R alpha-1 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 14 amino acid peptide near the center of human GFR alpha 1. The immunogen is located within amino acids 360 - 410 of GFR alpha 1.

Specificity

GFR alpha 1 antibody is predicted to not cross-react with other members of the GFR alpha family of proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free

Western Blot: GFR alpha-1/GDNF R alpha-1 AntibodyBSA Free [NBP1-77043]

Western Blot: GFR alpha-1/GDNF R alpha-1 AntibodyBSA Free [NBP1-77043]

Western Blot: GFR alpha-1/GDNF R alpha-1 Antibody [NBP1-77043] - Analysis of GFR alpha 1 in crude membrane fractions of human brain (B), liver (L), kidney (K), and spleen (S), respectively, with GFR alpha 1 antibody at 1 ug/mL
GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free [NBP1-77043] -

Immunocytochemistry/ Immunofluorescence: GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free [NBP1-77043] - Immunofluorescence of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 20 u/mL.

Red: GFR alpha 1 Antibody
Blue: DAPI staining
GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free

Immunohistochemistry: GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free [NBP1-77043] -

Immunohistochemistry: GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free [NBP1-77043] - Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 1 ug/mL.
GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free

Immunohistochemistry: GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free [NBP1-77043] -

Immunohistochemistry: GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free [NBP1-77043] - Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 2.5 ug/ml.

Applications for GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

1 ug/ml

Immunohistochemistry-Paraffin

1 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: GFR alpha-1/GDNF R alpha-1

Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nerves system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFRalpha, which is a cell surface receptor for GDNF. The cDNAs encoding GDNFRalpha from human, rat, chicken and mouse have been cloned recently. GDNFRalpha was also termed Ret ligand 1 (RETL1) or TGF-beta-related neurotrophic factor receptor 1 (TrnR1) and nominated as GFRalpha-1 recently. GFRalpha-1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFRalpha and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha 1

Alternate Names

GDNF R alpha-1, GFR alpha1, GFRa-1, GFRA1

Gene Symbol

Gfra1

UniProt

P56159

Additional GFR alpha-1/GDNF R alpha-1 Products

Product Documents for GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

GDNF Family

Citations for GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free

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  • Q: Could you please let me know if the following antibodies recognize an internal epitope : - NBP1-77043 (eptiope is between aa 369-382 : are they internal aa ?) - NB100-91781 (epitope is arround lysine 90 : is it an internal epitope ?)

    A: GFRA1 is 405 amino acidsd when full processed http://www.uniprot.org/uniprot/P56159). This processed form corresponds to amino acids 25-429, so these epitopdes correspond to being in the internal portion of GFRA1. This receptor is GPI-linked, so there is no transmembrane domain and therefore no cytoplasmic domain of this protein.

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