GILT/IFI30 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83366

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GILT/IFI30 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLEDQTQLLTLVCQLYQGKK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GILT/IFI30 Antibody - BSA Free

GILT/IFI30 Antibody

Immunohistochemistry-paraffin-GILT/IFI30 Antibody-NBP1-83366-

Immunohistochemistry-paraffin-GILT/IFI30 Antibody-NBP1-83366-Staining of human tonsil shows strong cytoplasmic positivity in a subset of non-germinal center cells.
GILT/IFI30 Antibody

ICC/IF-GILT/IFI30 Antibody-NBP1-83366-

ICC/IF-GILT/IFI30 Antibody-NBP1-83366- Staining of human cell line A-431 shows localization to cell junctions & vesicles.
GILT/IFI30 Antibody - BSA Free Western Blot: GILT/IFI30 Antibody - BSA Free [NBP1-83366]

Western Blot: GILT/IFI30 Antibody - BSA Free [NBP1-83366]

Analysis in human cell line RPMI-8226.
GILT/IFI30 Antibody - BSA Free Immunohistochemistry-Paraffin: GILT/IFI30 Antibody - BSA Free [NBP1-83366]

Immunohistochemistry-Paraffin: GILT/IFI30 Antibody - BSA Free [NBP1-83366]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
GILT/IFI30 Antibody - BSA Free Immunohistochemistry-Paraffin: GILT/IFI30 Antibody - BSA Free [NBP1-83366]

Immunohistochemistry-Paraffin: GILT/IFI30 Antibody - BSA Free [NBP1-83366]

Staining of human lung shows strong cytopalsmic positivity in macrophages.
GILT/IFI30 Antibody - BSA Free Immunohistochemistry-Paraffin: GILT/IFI30 Antibody - BSA Free [NBP1-83366]

Immunohistochemistry-Paraffin: GILT/IFI30 Antibody - BSA Free [NBP1-83366]

Staining of human cerebellum shows no positivity in Purkinje cells as expected.
GILT/IFI30 Antibody - BSA Free Immunohistochemistry-Paraffin: GILT/IFI30 Antibody - BSA Free [NBP1-83366]

Immunohistochemistry-Paraffin: GILT/IFI30 Antibody - BSA Free [NBP1-83366]

Analysis in human lung and skeletal muscle tissues using NBP1-83366 antibody. Corresponding IFI30 RNA-seq data are presented for the same tissues.

Applications for GILT/IFI30 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25 - 2 ug/mL

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-83366 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GILT/IFI30

IFI30 is encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. [provided by RefSeq]

Long Name

Gamma-Interferon-inducible Lysosomal Thiol Reductase

Alternate Names

IFI30, IP30, Legumaturain

Gene Symbol

IFI30

Additional GILT/IFI30 Products

Product Documents for GILT/IFI30 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GILT/IFI30 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GILT/IFI30 Antibody - BSA Free

Customer Reviews for GILT/IFI30 Antibody - BSA Free (1)

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  • GILT/IFI30 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: white adipose tissue, Liver, Brown adipose tissue and Adult Mouse Lung Tissue
    Species: Mouse
    Verified Customer | Posted 09/15/2020
    Different organs from WT and GILT KO mice
    GILT/IFI30 Antibody - BSA Free NBP1-83366

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Protocols

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