GIRK2 Antibody

Novus Biologicals | Catalog # NB100-74575

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GIRK2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SSKLNQHAELET corresponding to C-Terminus according to NP_002231.1.

Localization

Plasma membrane

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GIRK2 Antibody

Western Blot: GIRK2 Antibody [NB100-74575]

Western Blot: GIRK2 Antibody [NB100-74575]

Western Blot: GIRK2 Antibody [NB100-74575] - Staining of Human Brain (Substantia Nigra) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
GIRK2 Antibody

Western Blot: GIRK2 Antibody [NB100-74575] -

Hypothalamic neuronal activity under 5-HT1Areceptor agonist treatment. After fluoxetine injection for 4 weeks, hypothalamic CRH neuronal activity (CRH, CRH_R2 and c-Fos) before and after challenge with 8-OH-DPAT was evaluated by immunofluorescent staining (A) and western blotting (B) analysis. In the hypothalamic plasma membrane, the protein expression levels of the 5-HT1A receptors, GIRK1 and GIRK2 were measured (C). The protein expression levels of BDNF, phosphorylated ERK1/2 and CREB in whole hypothalamic lysate were analyzed (D). The data are expressed as the mean +/- SD (n = 4/group) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38191373), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for GIRK2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1-3 ug/ml
Application Notes
WB: Approx. 48 kDa band observed in human brain (hippocampus and substantia nigra) lysates (calculated MW of 48.5 kDa band according to NP_002231.1). Use in IHC, ICC/IF reported in scientific literature (PMID: 28398344).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GIRK2

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and may be involved in the regulation of insulin secretion by glucose. It associates with two other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex.

Long Name

G protein-activated inward rectifier potassium channel 2

Alternate Names

BIR1, GIRK-2, KATP-2, KATP2, KCNJ6, KCNJ7

Entrez Gene IDs

3763 (Human); 16522 (Mouse); 25743 (Rat)

Gene Symbol

KCNJ6

UniProt

P48051 (Human)

Additional GIRK2 Products

Product Documents for GIRK2 Antibody

Certificate of Analysis

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Product Specific Notices for GIRK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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