Key Product Details

Species Reactivity

Mammal

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 53

Format

BSA Free
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Product Specifications

Immunogen

Synthetic GLP-1(9-18)

Epitope

The free N-terminus of GLP-1(9-36)amide and GLP-1(9-37)

Specificity

Based on inhibition ELISA, It cross-reacts

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Novus Biologicals Mouse Glucagon Antibody (53) - BSA Free (NBP1-05184) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Glucagon Antibody (53) - BSA Free

Glucagon Antibody (53) - BSA Free [NBP1-05184] - BSA Free [NBP1-05184]

Applications for Glucagon Antibody (53) - BSA Free

Application
Recommended Usage

ELISA

1:6000

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP1-05184 binds free GLP-1(9-36)amide in solution. NBP1-05184 has higher affinity for GLP-1(9-36)amide than NBP2-23591 as determined by inhibition. WB: NBP1-05184 can be used in Western blotting.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Glucagon

Glucagon-like peptide-1(9-36)amide and GLP-1(9-37) are the forms of GLP-1 that result from the rapid degradation of the active forms of the peptide (GLP-1(7-36)amide and GLP-1(7-37)) by the enzyme dipeptidyl peptidase-IV (DPP-IV, also known as CD26 or adenosine deaminase binding protein). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Alternate Names

GCG, GRPP

Gene Symbol

GCG

Additional Glucagon Products

Product Documents for Glucagon Antibody (53) - BSA Free

Certificate of Analysis

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Product Specific Notices for Glucagon Antibody (53) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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