Glucagon Antibody (K79bB10)
Novus Biologicals | Catalog # NB600-1506
![Immunohistochemistry: Glucagon Antibody (K79bB10) [NB600-1506]](https://resources.rndsystems.com/images/products/Glucagon-Antibody-K79bB10-Immunohistochemistry-NB600-1506-img0001.jpg "Immunohistochemistry: Glucagon Antibody (K79bB10) [NB600-1506]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Canine, Feline, Guinea Pig, Rabbit
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Dot Blot, Radioimmunoassay
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # K79bB10
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Product Specifications
Immunogen
Polymerized porcine glucagon.
Reactivity Notes
Not yet tested in other species.
Localization
Secreted
Specificity
The antibody reacts specifically against pancreatic glucagon and exhibits only very weak cross-reaction with gut glucagon (enteroglucagon).Glucagon (K79bB10)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Glucagon Antibody (K79bB10)
Immunohistochemistry: Glucagon Antibody (K79bB10) [NB600-1506]
Immunohistochemistry: Glucagon Antibody (K79bB10) [NB600-1506] - Mouse pancreatic islet alpha cells were stained using Monoclonal Anti-Glucagon, clone no. K79bB10, Anti-Mouse IgG-peroxidase and AEC substrate (red). The tissue was counterstained with hematoxylin (blue).Applications for Glucagon Antibody (K79bB10)
Application
Recommended Usage
Dot Blot
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:2000
Radioimmunoassay
1:100-1:2000
Application Notes
Reacts with pancreatic glucagon. RIA: Use at an assay dependent dilution, affinity constant of 6.1 x 10^8 L/M, reacts with pancreatic glucagon.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Preservative
0.09% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Glucagon
Additional Glucagon Products
Product Documents for Glucagon Antibody (K79bB10)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Glucagon Antibody (K79bB10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Glucagon Antibody (K79bB10)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Glucagon Antibody (K79bB10)
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Q: Would you be able to confirm that product NB600-1506 is raised against polymerized glucagon w/ the sequence (HSQGTFTSDYSKYLDSRRAQDFVQWLMNT)?
A: Product NB600-1506 was raised against the full length porcine polymerized glucagon sequence. That sequence shares 100% homology with the sequence you have provided in your message.
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