Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free

Novus Biologicals | Catalog # NB100-236

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunoblotting

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunoblotting

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 50 and 100 of human Glucose-6-Phosphate Dehydrogenase using the numbering given in entry NP_000393.2 (GeneID 2539).

Marker

Cytosol Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236]

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236]

Glucose-6-Phosphate-Dehydrogenase-Antibody-Western-Blot-NB100-236-img0009.jpg
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236]

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236]

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Detection of Human and Mouse G6PD by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G6PD antibody NB100-236 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Glucose 6 Phosphate Dehydrogenase Antibody

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] -

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Dysregulation of Antioxidant proteins in response to chronic endurance exercise in aged Nrf2−/− mice hearts. Antioxidant protein expression (GPX1, CAT, G6PD, & SOD1) were determined using immunoblotting with specific antibodies. The relative intensity signals were quantified using ImageJ software & normalized to GAPDH intensity & represented as histogram. Experiments were analyzed using one way ANOVA followed by Tukey multiple comparison tests & differences between the means were considered statistically significant if P < 0.05 (* vs. WT-SED, # vs. Nrf2−/−-SED, $ vs. WT CEE). Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fphys.2017.00268/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Glucose 6 Phosphate Dehydrogenase Antibody

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] -

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Effect of prolonged moderate exercise on Nrf2/ARE-Antioxidants in the aging heart.Representative immunoblots of cytosolic extracts from the hearts of young & old mice under basal conditions & following 6-weeks of moderate exercise training (MET). Protein blots were probed with respective antibodies as indicated. Individual lanes represent separate animals (n = 4–6/group). A. Analysis of nuclear Nrf2 in young & old mice subjected to MET. In sedentary mice, Nrf2 protein levels were decreased significantly in old when compared to young (*p<0.05). Following MET, nuclear Nrf2 levels were significantly increased in old mice to levels equivalent to those of young mice (#, $p<0.05 in MET vs. respective basal). (B) Densitometry analysis of respective protein signals were performed using Image-J & expressed relative to mean values of the sedentary-young group. Under basal conditions, a significant decrease in the protein levels of GSR, G6PD, NQO1, catalase, GPX1 & GCS were observed in the heart tissues of old when compared to young mice. Following 6-weeks of moderate exercise, most of the antioxidants were significantly (*p<0.05) upregulated or stabilized in the aging heart. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0045697), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:500-1:2500
Application Notes
Use in IHC-Frozen reported in scientific literature (PMID: 17693254). Use in Immunoblotting reported in scientific literature (PMID 28515695).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: G6PD

Glucose-6-phosphate dehydrogenase (G6PD) is a cytosolic enzyme that catalyzes the conversion of glucose-6-phosphate into 6-phosphgluconolactone and maintains the levels of the electron donor, NADPH. G6PD is an x-linked housekeeping gene whose deficiency can result in neonatal jaundice, acute hemolysis, or chronic non-spherocytic hemolytic anemia.

Long Name

Glucose-6-Phosphate Dehydrogenase

Alternate Names

G6PDH

Gene Symbol

G6PD

Additional G6PD Products

Product Documents for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Citations for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free

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Protocols

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