Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free
Novus Biologicals | Catalog # NB100-236
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunoblotting
Cited:
Immunohistochemistry-Frozen, Western Blot, Immunoblotting
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 50 and 100 of human Glucose-6-Phosphate Dehydrogenase using the numbering given in entry NP_000393.2 (GeneID 2539).
Marker
Cytosol Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236]
Glucose-6-Phosphate-Dehydrogenase-Antibody-Western-Blot-NB100-236-img0009.jpgWestern Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236]
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Detection of Human and Mouse G6PD by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G6PD antibody NB100-236 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] -
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Dysregulation of Antioxidant proteins in response to chronic endurance exercise in aged Nrf2−/− mice hearts. Antioxidant protein expression (GPX1, CAT, G6PD, & SOD1) were determined using immunoblotting with specific antibodies. The relative intensity signals were quantified using ImageJ software & normalized to GAPDH intensity & represented as histogram. Experiments were analyzed using one way ANOVA followed by Tukey multiple comparison tests & differences between the means were considered statistically significant if P < 0.05 (* vs. WT-SED, # vs. Nrf2−/−-SED, $ vs. WT CEE). Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fphys.2017.00268/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] -
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Effect of prolonged moderate exercise on Nrf2/ARE-Antioxidants in the aging heart.Representative immunoblots of cytosolic extracts from the hearts of young & old mice under basal conditions & following 6-weeks of moderate exercise training (MET). Protein blots were probed with respective antibodies as indicated. Individual lanes represent separate animals (n = 4–6/group). A. Analysis of nuclear Nrf2 in young & old mice subjected to MET. In sedentary mice, Nrf2 protein levels were decreased significantly in old when compared to young (*p<0.05). Following MET, nuclear Nrf2 levels were significantly increased in old mice to levels equivalent to those of young mice (#, $p<0.05 in MET vs. respective basal). (B) Densitometry analysis of respective protein signals were performed using Image-J & expressed relative to mean values of the sedentary-young group. Under basal conditions, a significant decrease in the protein levels of GSR, G6PD, NQO1, catalase, GPX1 & GCS were observed in the heart tissues of old when compared to young mice. Following 6-weeks of moderate exercise, most of the antioxidants were significantly (*p<0.05) upregulated or stabilized in the aging heart. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0045697), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
1:500-1:2500
Application Notes
Use in IHC-Frozen reported in scientific literature (PMID: 17693254). Use in Immunoblotting reported in scientific literature (PMID 28515695).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: G6PD
Long Name
Glucose-6-Phosphate Dehydrogenase
Alternate Names
G6PDH
Gene Symbol
G6PD
Additional G6PD Products
Product Documents for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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