Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89804

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all G6PD Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FQGDAFHQSDTHIFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKATPEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALH

Marker

Cytosol Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free

Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free [NBP1-89804]

Immunocytochemistry/ Immunofluorescence: Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free [NBP1-89804]

Staining of human cell line U-2 OS shows positivity in cytoplasm.
Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free [NBP1-89804]

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free [NBP1-89804]

Analysis in human testis and skeletal muscle tissues using NBP1-89804 antibody. Corresponding G6PD RNA-seq data are presented for the same tissues.
Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free [NBP1-89804]

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free [NBP1-89804]

Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free [NBP1-89804]

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free [NBP1-89804]

Staining of human liver shows moderate to strong cytoplasmic positivity in Kupffer cells.
Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free [NBP1-89804]

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free [NBP1-89804]

Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.
Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free [NBP1-89804]

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free [NBP1-89804]

Staining of human spleen shows moderate cytoplasmic positivity in lymphoid cells.
Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free [NBP1-89804]

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free [NBP1-89804]

Staining of human colon shows moderate cytoplasmic positivity in a subset of lymphoid cells.
Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free [NBP1-89804]

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free [NBP1-89804]

Analysis in human cell lines A-549 and HEK293 using Anti-G6PD antibody. Corresponding G6PD RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804]

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804]

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Analysis using Anti-G6PD antibody NBP1-89804 (A) shows similar pattern to independent antibody NBP1-89805 (B).

Applications for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: G6PD

Glucose-6-phosphate dehydrogenase (G6PD) is a cytosolic enzyme that catalyzes the conversion of glucose-6-phosphate into 6-phosphgluconolactone and maintains the levels of the electron donor, NADPH. G6PD is an x-linked housekeeping gene whose deficiency can result in neonatal jaundice, acute hemolysis, or chronic non-spherocytic hemolytic anemia.

Long Name

Glucose-6-Phosphate Dehydrogenase

Alternate Names

G6PDH

Entrez Gene IDs

2539 (Human)

Gene Symbol

G6PD

UniProt

P11413

Additional G6PD Products

Product Documents for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

Citations for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free

Customer Reviews for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free

There are currently no reviews for this product. Be the first to review Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free and earn rewards!

Have you used Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies