GluR1 [p Ser845] Antibody - Azide Free

Novus Biologicals | Catalog # NB300-171

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse

Predicted:

Human (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all GluR1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser845 conjugated to KLH. Accession # P19490

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24212670)

Modification

p Ser845

Localization

Cell Membrane

Specificity

Specific for endogenous levels of the ~100 kDa GluR1 protein phosphorylated at Ser845. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit GluR1 [p Ser845] Antibody - Azide Free (NB300-171) is a polyclonal antibody validated for use in IHC, WB and Simple Western. Anti-GluR1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for GluR1 [p Ser845] Antibody - Azide Free

Western Blot: GluR1 [p Ser845] Antibody [NB300-171]

Western Blot: GluR1 [p Ser845] Antibody [NB300-171]

Western Blot: GluR1 [p Ser845] Antibody [NB300-171] - Rat hippocampal lysate showing specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser845 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the GluR1 Ser845 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Applications for GluR1 [p Ser845] Antibody - Azide Free

Application
Recommended Usage

Immunohistochemistry

1:1000

Immunohistochemistry-Frozen

1:1000

Simple Western

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GluR1

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The GRIA1 belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Each of the members (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing. The receptor subunits encoded by each isoform vary in their signal transduction properties. The isoform presented here is the flop isoform. In situ hybridization experiments showed that human GRIA1 mRNA is present in granule and pyramidal cells in the hippocampal formation.

Long Name

Glutamate Receptor 1

Alternate Names

GluA1, GLURA, GRIA1

Entrez Gene IDs

2890 (Human); 14810 (Mouse); 50592 (Rat)

Gene Symbol

GRIA1

UniProt

P19490 (Rat)

Additional GluR1 Products

Product Documents for GluR1 [p Ser845] Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for GluR1 [p Ser845] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GluR1 [p Ser845] Antibody - Azide Free

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Protocols

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FAQs for GluR1 [p Ser845] Antibody - Azide Free

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  • Q: Could you please provide the immunogen sequence for catalog number NB300-171 at your earliest convenience? If the exact sequence is not available, could you please tell me a broader range of amino acids or the region of the peptide from which it is derived?

    A: The immunogen for this one was taken from within the 30 amino acids that surround the serine 845.

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