Glut3 Antibody (14B1) - BSA Free

Novus Biologicals | Catalog # NBP3-26317

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 14B1 expressed in HEK293

Format

BSA Free
View all Glut3 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Glut3 [UniProt P11169]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glut3 Antibody (14B1) - BSA Free

Glut3 Antibody (14B1)

Immunohistochemistry: Glut3 Antibody (14B1) [NBP3-26317] -

Immunohistochemistry: Glut3 Antibody (14B1) [NBP3-26317] - Image of Glut3 Antibody (14B1) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Glut3 Antibody (14B1)

Immunohistochemistry: Glut3 Antibody (14B1) [NBP3-26317] -

Immunohistochemistry: Glut3 Antibody (14B1) [NBP3-26317] - Image of Glut3 Antibody (14B1) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Glut3 Antibody (14B1)

Immunohistochemistry: Glut3 Antibody (14B1) [NBP3-26317] -

Immunohistochemistry: Glut3 Antibody (14B1) [NBP3-26317] - Image of Glut3 Antibody (14B1) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Glut3 Antibody (14B1)

Flow Cytometry: Glut3 Antibody (14B1) [NBP3-26317] -

Flow Cytometry: Glut3 Antibody (14B1) [NBP3-26317] - Overlay Peak curve showing MCF7 cells stained with Glut3 Antibody (14B1) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Glut3 Antibody (14B1)

Western Blot: Glut3 Antibody (14B1) [NBP3-26317]

Western Blot: Glut3 Antibody (14B1) [NBP3-26317] -Western Blot
Positive WB detected in: 293 whole cell lysate (20µg), JK whole cell lysate (20µg), Hela whole cell lysate (20µg), HepG2 whole cell lysate (20µg), SY5Y whole cell lysate (20µg)
All lanes: SLC2A3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa

Applications for Glut3 Antibody (14B1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Glut3

Glucose is the major source of our energy and there are numerous isoforms of the glucose transporter in mammals, including Glut1, Glut2, Glut3, Glut4, Glut5, Glut6, Glut7, Glut8 and Glut9. The Glut5 gene located on the short arm of human chromosome 1 encodes a 501-amino acid facilitative glucose transporter. Glut5 mRNA is highly expressed in small intestine and to a lesser extent in kidney, skeletal muscle and adipose tissue. Glut5 plays a critical role in fructose absorption in the small intestine and its expression is highly induced when exposed to a fructose-enriched diet. Glut5 transporter expressed in human skeletal muscle is specifically localized to the plasma membrane, where it participates in regulating hexose transfer across the sarcolemma. Glut8, a novel glucose transporter-like protein, exhibits significant sequence similarity with the other members of sugar transporter family. Glut8 comprises 12 putative membrane-spanning helices and several conserved motifs, which are important for transport activity. In human tissues, Glut8 is predominantly expressed in testis and, to a lesser extent, in most other tissues including skeletal muscle, heart, small intestine and brain. In addition, the Glut8 glucose transport facilitator has a hormonally regulated testicular function.

Long Name

Glucose Transporter Type 3

Alternate Names

SLC2A3

Gene Symbol

SLC2A3

Additional Glut3 Products

Product Documents for Glut3 Antibody (14B1) - BSA Free

Certificate of Analysis

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Product Specific Notices for Glut3 Antibody (14B1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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