Glut5 Antibody (OTI9F3)

Novus Biologicals | Catalog # NBP1-47980

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI9F3
View all Glut5 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SLC2A5 (NP_003030) produced in HEK293T cell.

Specificity

This antibody is specific for Homo sapiens solute carrier family 2 (facilitated glucose/fructose transporter), member 5 (SLC2A5), transcript variant 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

54.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glut5 Antibody (OTI9F3)

Western Blot: Glut5 Antibody (OTI9F3) [NBP1-47980]

Western Blot: Glut5 Antibody (OTI9F3) [NBP1-47980]

Western Blot: Glut5 Antibody (OTI9F3) [NBP1-47980] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti- GLUT5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980]

Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980]

Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980] - Human adult kidney tissue section stained with Glut5 antibody at 1:200. Secondary antibody at 1:300. IHC-P image submitted by a verified customer review.
Flow Cytometry: Glut5 Antibody (OTI9F3) [NBP1-47980]

Flow Cytometry: Glut5 Antibody (OTI9F3) [NBP1-47980]

Flow Cytometry: Glut5 Antibody (OTI9F3) [NBP1-47980] - Analysis of Jurkat cells, using anti-GLUT5 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: Glut5 Antibody (OTI9F3) [NBP1-47980]

Western Blot: Glut5 Antibody (OTI9F3) [NBP1-47980]

Western Blot: Glut5 Antibody (OTI9F3) [NBP1-47980] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLUT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti- GLUT5.
Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980]

Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980]

Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980] - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980) Image using the Azide and BSA Free format of this antibody.
Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980]

Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980]

Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980] - Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980) Image using the Azide and BSA Free format of this antibody.
Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980]

Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980]

Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980] - Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980) Image using the Azide and BSA Free format of this antibody.
Flow Cytometry: Glut5 Antibody (OTI9F3) [NBP1-47980]

Flow Cytometry: Glut5 Antibody (OTI9F3) [NBP1-47980]

Flow Cytometry: Glut5 Antibody (OTI9F3) [NBP1-47980] - Analysis of Hela cells, using anti-GLUT5 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunoprecipitation: Glut5 Antibody (OTI9F3) [NBP1-47980]

Immunoprecipitation: Glut5 Antibody (OTI9F3) [NBP1-47980]

Immunoprecipitation: Glut5 Antibody (OTI9F3) [NBP1-47980] - Immunoprecipitation(IP) of Glut5 by using monoclonal anti-Glut5 antibodies (Negative control: IP without adding anti-Glut5 antibody). For each experiment, 500ul of DDK tagged Glut5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-Glut5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody. Image using the Azide and BSA Free format of this antibody.

Applications for Glut5 Antibody (OTI9F3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

2ug/500ul

Western Blot

1:500-1000

Reviewed Applications

Read 1 review rated 5 using NBP1-47980 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1% BSA, 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glut5

Cytochalasin B-sensitive carrier. Seems to function primarily as a fructose transporter.

Long Name

Glucose Transporter Type 5

Alternate Names

SLC2A5

Entrez Gene IDs

6518 (Human)

Gene Symbol

SLC2A5

Additional Glut5 Products

Product Documents for Glut5 Antibody (OTI9F3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Glut5 Antibody (OTI9F3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes
Glucose Homeostasis
Metabolism

Customer Reviews for Glut5 Antibody (OTI9F3) (1)

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  • Glut5 Antibody (OTI9F3)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Adult kidney
    Species: Human
    Verified Customer | Posted 12/06/2021
    Staining in kidney tissue.
    Primary antibody dilution 1:200. Secondary antibody dilution 1:300.
    Glut5 Antibody (OTI9F3) NBP1-47980

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Protocols

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