Glut5 Antibody (OTI9F3) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71285

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI9F3

Format

Azide and BSA Free
View all Glut5 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SLC2A5 (NP_003030) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

54.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glut5 Antibody (OTI9F3) - Azide and BSA Free

Western Blot: Glut5 Antibody (OTI9F3)Azide and BSA Free [NBP2-71285]

Western Blot: Glut5 Antibody (OTI9F3)Azide and BSA Free [NBP2-71285]

Western Blot: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-Glut5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285]

Immunohistochemistry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285]

Immunohistochemistry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980)
Flow Cytometry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285]

Flow Cytometry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285]

Flow Cytometry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Flow cytometric Analysis of Jurkat cells, using anti-Glut5 antibody (NBP1-47980),(Red), compared to a nonspecific negative control antibody,(Blue).
Western Blot: Glut5 Antibody (OTI9F3)Azide and BSA Free [NBP2-71285]

Western Blot: Glut5 Antibody (OTI9F3)Azide and BSA Free [NBP2-71285]

Western Blot: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glut5 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glut5.
Immunohistochemistry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285]

Immunohistochemistry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285]

Immunohistochemistry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980)
Immunohistochemistry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285]

Immunohistochemistry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285]

Immunohistochemistry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980)
Flow Cytometry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285]

Flow Cytometry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285]

Flow Cytometry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Flow cytometric Analysis of Hela cells, using anti-Glut5 antibody (NBP1-47980),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunoprecipitation: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285]

Immunoprecipitation: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285]

Immunoprecipitation: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Immunoprecipitation(IP) of Glut5 by using monoclonal anti-Glut5 antibodies (Negative control: IP without adding anti-Glut5 antibody). For each experiment, 500ul of DDK tagged Glut5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-Glut5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Applications for Glut5 Antibody (OTI9F3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:50

Immunoprecipitation

2ug/500ul

Western Blot

1:500-1000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glut5

Facilitative simple sugar uptake by mammalian cells is mediated by a family of glucose transporters. Gluts are predicted to contain 12 transmembrane domains with both amino- and carboxyl-termini located within the cytosol. The individual Gluts differ in their tissue distribution, substrate specificity, kinetic properties, and intracellular localization. These functional differences allow members of the Glut family to finely regulate whole-body glucose homeostasis.

Long Name

Glucose Transporter Type 5

Alternate Names

SLC2A5

Gene Symbol

SLC2A5

Additional Glut5 Products

Product Documents for Glut5 Antibody (OTI9F3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Glut5 Antibody (OTI9F3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes
Glucose Homeostasis
Metabolism

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Protocols

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FAQs

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