Glutamate Dehydrogenase 2/GLUD2 Antibody

Novus Biologicals | Catalog # NBP2-16678

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Glutamate Dehydrogenase 2/GLUD2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GLUD1+2. The exact sequence is proprietary.

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutamate Dehydrogenase 2/GLUD2 Antibody

Western Blot: Glutamate Dehydrogenase 2/GLUD2 Antibody [NBP2-16678]

Western Blot: Glutamate Dehydrogenase 2/GLUD2 Antibody [NBP2-16678]

Western Blot: Glutamate Dehydrogenase 2/GLUD2 Antibody [NBP2-16678] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutamate Dehydrogenase 2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Glutamate Dehydrogenase 2/GLUD2 Antibody [NBP2-16678]

Immunohistochemistry-Paraffin: Glutamate Dehydrogenase 2/GLUD2 Antibody [NBP2-16678]

Immunohistochemistry-Paraffin: Glutamate Dehydrogenase 2/GLUD2 Antibody [NBP2-16678] - Analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.
Western Blot: Glutamate Dehydrogenase 2/GLUD2 Antibody [NBP2-16678]

Western Blot: Glutamate Dehydrogenase 2/GLUD2 Antibody [NBP2-16678]

Western Blot: Glutamate Dehydrogenase 2/GLUD2 Antibody [NBP2-16678] - A: 293T B: A431 C: HeLa D: HepG2.
Western Blot: Glutamate Dehydrogenase 2/GLUD2 Antibody [NBP2-16678]

Western Blot: Glutamate Dehydrogenase 2/GLUD2 Antibody [NBP2-16678]

Western Blot: Glutamate Dehydrogenase 2/GLUD2 Antibody [NBP2-16678] - A: Mouse Testis.
Glutamate Dehydrogenase 2/GLUD2 Antibody

Western Blot: Glutamate Dehydrogenase 2/GLUD2 Antibody [NBP2-16678] -

Drosophila tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GLUD1 + GLUD2 antibody (NBP2-16678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Glutamate Dehydrogenase 2/GLUD2 Antibody

Immunocytochemistry/ Immunofluorescence: Glutamate Dehydrogenase 2/GLUD2 Antibody [NBP2-16678] -

GLUD1 + GLUD2 antibody detects GLUD1 + GLUD2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GLUD1 + GLUD2 protein stained by GLUD1 + GLUD2 antibody (NBP2-16678) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for Glutamate Dehydrogenase 2/GLUD2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glutamate Dehydrogenase 2/GLUD2

Glutamate dehydrogenase (EC 1.4.1.3) catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate using NAD and/or NADP as cofactors. See also GLUD1 (MIM 138130).[supplied by OMIM]

Alternate Names

GDH2, GLUD2, GLUDP1

Gene Symbol

GLUD2

UniProt

P49448

Additional Glutamate Dehydrogenase 2/GLUD2 Products

Product Documents for Glutamate Dehydrogenase 2/GLUD2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Glutamate Dehydrogenase 2/GLUD2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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