Glutaminase Antibody (3211) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43762

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse, Rat, Porcine, Bovine, Ovine

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3211

Format

Azide and BSA Free
View all Glutaminase Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Glutaminase (GAC). The exact sequence is proprietary.

Specificity

Specific for GAC but not KGA isoform.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutaminase Antibody (3211) - Azide and BSA Free

Western Blot: Glutaminase Antibody (3211) [NBP2-43762]

Western Blot: Glutaminase Antibody (3211) [NBP2-43762]

Western Blot: Glutaminase Antibody (3211) [NBP2-43762] - Analysis of various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody [3211] diluted at a dilution of 1:1000.
Glutaminase Antibody (3211) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody (3211) [NBP2-43762] -

Glutaminase C antibody [GT3211] detects Glutaminase C protein at mitochondria by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutaminase C protein stained by Glutaminase C antibody [GT3211] (NBP2-43762) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
Glutaminase Antibody (3211) - Azide and BSA Free

Immunohistochemistry-Paraffin: Glutaminase Antibody (3211) [NBP2-43762] -

Glutaminase C antibody [GT3211] detects Glutaminase C protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Glutaminase C antibody [GT3211] (NBP2-43762) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Glutaminase Antibody (3211) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glutaminase

Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

Long Name

Glutaminase Kidney Isoform, Mitochondrial

Alternate Names

GLS, K-Glutaminase, L-Glutamine Amidohydrolase

Gene Symbol

GLS

Additional Glutaminase Products

Product Documents for Glutaminase Antibody (3211) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Glutaminase Antibody (3211) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Glutaminase Antibody (3211) - Azide and BSA Free

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Protocols

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FAQs for Glutaminase Antibody (3211) - Azide and BSA Free

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  • Q: I was wondering what the specificity of this enzyme is in regards to the glutaminase isoforms gls1 and gls2. Ideally, this would have no cross reactivity with gls2.

    A:

    Our Glutaminase antibody NBP2-43762 was made to an immunogen that falls between aa 551-669 of the human glutaminase C (GAC) protein: http://www.uniprot.org/uniprot/O94925#sequences. This antibody has been found to not cross react against the canonical (KGA) sequence for GLS1. It has not yet been tested for cross reactivity against the GLS2 protein. Based on an alignment of the immunogen range and the GLS2 sequence, there does seem to be some areas with significant homology, so there is a chance it could react.

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