Glutamine Synthetase Antibody (1055) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43646

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (96%), Canine (97%), Chimpanzee (99%), Porcine (96%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Whole-Mount, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry Whole-Mount

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1055

Format

Azide and BSA Free
View all Glutamine Synthetase Primary Antibodies »

Product Specifications

Immunogen

Full length human Glutamine Synthetase Recombinant protein.

Reactivity Notes

Xenopus laevis (85%), Chicken (88%),

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutamine Synthetase Antibody (1055) - Azide and BSA Free

Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646]

Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646]

Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [1055] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646]

Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646]

Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [1055] dilution: 1:1000
Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646]

Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646]

Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of A. 30 ug U87-MG whole cell lysate/extract B. 30 ug SK-N-SH whole cell lysate/extract C. 30 ug IMR32 whole cell lysate/extract D. 30 ug SK-N-AS whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [1055] dilution: 1:1000
Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646]

Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646]

Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of human hepatoma. Glutamine synthetase antibody [1055] dilution: 1:500.
Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646]

Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646]

Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of mouse skin. Glutamine synthetase antibody [1055] dilution: 1:500.
Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646]

Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646]

Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of rat hind brain. Glutamine synthetase antibody [1055] dilution: 1:500.
Immunohistochemistry-Frozen: Glutamine Synthetase Antibody (1055) [NBP2-43646]

Immunohistochemistry-Frozen: Glutamine Synthetase Antibody (1055) [NBP2-43646]

Immunohistochemistry-Frozen: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Glutamine synthetase antibody [1055] detects Glutamine synthetase protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Glutamine synthetase antibody [1055] diluted at 1:500. Blue: DAPI
Glutamine Synthetase Antibody (1055) - Azide and BSA Free

Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] -

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:5000.
Glutamine Synthetase Antibody (1055) - Azide and BSA Free

Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:500.
Glutamine Synthetase Antibody (1055) - Azide and BSA Free

Immunohistochemistry-Frozen: Glutamine Synthetase Antibody (1055) [NBP2-43646] -

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:250.
Red: PKC alpha protein stained by PKC alpha antibody [GT1876] diluted at 1:250.
Blue: Fluoroshield with DAPI.
Glutamine Synthetase Antibody (1055) - Azide and BSA Free

Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] -

Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (NBP2-43646) diluted at 1:1000.
Glutamine Synthetase Antibody (1055) - Azide and BSA Free

Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] -

Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:5000.
Glutamine Synthetase Antibody (1055) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody (1055) [NBP2-43646] -

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytoplasm by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:250.
Red: GFAP, stained by GFAP antibody diluted at 1:250.
Blue: Fluoroshield with DAPI.

Applications for Glutamine Synthetase Antibody (1055) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry Whole-Mount

Reported in scientific literature (PMID: 31337815)

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glutamine Synthetase

Glutamine synthetase is part of the glutamine synthetase family. Specifically, Glutamine synthetase catalyzes the condensation of glutamate and ammonia to form glutamine, during the metabolism of nitrogen. Ammonia incorporation in animals occurs through the actions of glutamate dehydrogenase and glutamine synthase. Glutamate plays the central role in mammalian nitrogen flow, serving as both a nitrogen donor and nitrogen acceptor.

Glutamine synthetase antibodies are useful tools for studying nitrogen metabolism and regulation.

Alternate Names

cell proliferation-inducing protein 59, EC 4.1.1.15, EC 6.3.1.2, GLNSPIG59, Glutamate decarboxylase, glutamate-ammonia ligase, Glutamate--ammonia ligase, glutamate-ammonia ligase (glutamine synthase), glutamine synthase, glutamine synthetase, GS, PIG43, proliferation-inducing protein 43

Gene Symbol

GLUL

Additional Glutamine Synthetase Products

Product Documents for Glutamine Synthetase Antibody (1055) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Glutamine Synthetase Antibody (1055) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Glutamine Synthetase Antibody (1055) - Azide and BSA Free

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Protocols

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FAQs

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