Glutamine Synthetase Antibody (7711) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43826

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7711

Format

Azide and BSA Free
View all Glutamine Synthetase Primary Antibodies »

Product Specifications

Immunogen

Full length human Glutamine Synthetase Recombinant protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutamine Synthetase Antibody (7711) - Azide and BSA Free

Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826]

Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826]

Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (7711) diluted at 1:500.
Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826]

Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826]

Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [7711] dilution: 1:1000
Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826]

Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826]

Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody diluted at 1:1000.
Glutamine Synthetase Antibody (7711)

Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody (7711) [NBP2-43826] -

Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Glutamine Synthetase antibody [GT7711] detects Glutamine Synthetase protein in glial cells by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron & glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutamine Synthetase stained by Glutamine Synthetase antibody [GT7711] (NBP2-43826) diluted at 1:500.
Red: Tau, a Tau marker, stained by Tau antibody diluted at 1:500.
Blue: Fluoroshield with DAPI.
Glutamine Synthetase Antibody (7711)

Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] -

Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine Synthetase antibody [GT7711] (NBP2-43826) diluted at 1:250000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Glutamine Synthetase Antibody (7711)

Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (7711) [NBP2-43826] -

Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Glutamine Synthetase antibody [GT7711] detects Glutamine Synthetase protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Glutamine Synthetase stained by Glutamine Synthetase antibody [GT7711] (NBP2-43826) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Glutamine Synthetase Antibody (7711) - Azide and BSA Free

Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (7711) [NBP2-43826] -

Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Glutamine Synthetase Antibody (7711) - Azide and BSA Free

Immunohistochemistry-Frozen: Glutamine Synthetase Antibody (7711) [NBP2-43826] -

Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:250.
Red: PKC alpha protein stained by PKC alpha antibody [GT1876] diluted at 1:250.
Blue: Fluoroshield with DAPI.
Glutamine Synthetase Antibody (7711) - Azide and BSA Free

Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Glutamine Synthetase Antibody (7711) - Azide and BSA Free

Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (7711) [NBP2-43826] -

Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:200.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Glutamine Synthetase Antibody (7711) - Azide and BSA Free

Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (7711) [NBP2-43826] -

Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Glutamine Synthetase Antibody (7711) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glutamine Synthetase

Glutamine synthetase is part of the glutamine synthetase family. Specifically, Glutamine synthetase catalyzes the condensation of glutamate and ammonia to form glutamine, during the metabolism of nitrogen. Ammonia incorporation in animals occurs through the actions of glutamate dehydrogenase and glutamine synthase. Glutamate plays the central role in mammalian nitrogen flow, serving as both a nitrogen donor and nitrogen acceptor.

Glutamine synthetase antibodies are useful tools for studying nitrogen metabolism and regulation.

Alternate Names

cell proliferation-inducing protein 59, EC 4.1.1.15, EC 6.3.1.2, GLNSPIG59, Glutamate decarboxylase, glutamate-ammonia ligase, Glutamate--ammonia ligase, glutamate-ammonia ligase (glutamine synthase), glutamine synthase, glutamine synthetase, GS, PIG43, proliferation-inducing protein 43

Gene Symbol

GLUL

Additional Glutamine Synthetase Products

Product Documents for Glutamine Synthetase Antibody (7711) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Glutamine Synthetase Antibody (7711) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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