Glutamine Synthetase Antibody (8D7) - BSA Free

Novus Biologicals | Catalog # NBP2-60240

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 8D7

Format

BSA Free
View all Glutamine Synthetase Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Glutamine Synthetase (1-373aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Glutamine Synthetase Antibody (8D7) - BSA Free

Western Blot: Glutamine Synthetase Antibody (8D7)BSA Free [NBP2-60240]

Western Blot: Glutamine Synthetase Antibody (8D7)BSA Free [NBP2-60240]

Western Blot: Glutamine Synthetase Antibody (8D7) [NBP2-60240] - The mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Glutamine Synthetase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse brain tissue lysateLane 2.: Mouse liver tissue lysate
Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody (8D7) - BSA Free [NBP2-60240]

Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody (8D7) - BSA Free [NBP2-60240]

Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody (8D7) [NBP2-60240] - Staining in HeLa cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Applications for Glutamine Synthetase Antibody (8D7) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glutamine Synthetase

Glutamine synthetase is part of the glutamine synthetase family. Specifically, Glutamine synthetase catalyzes the condensation of glutamate and ammonia to form glutamine, during the metabolism of nitrogen. Ammonia incorporation in animals occurs through the actions of glutamate dehydrogenase and glutamine synthase. Glutamate plays the central role in mammalian nitrogen flow, serving as both a nitrogen donor and nitrogen acceptor.

Glutamine synthetase antibodies are useful tools for studying nitrogen metabolism and regulation.

Alternate Names

cell proliferation-inducing protein 59, EC 4.1.1.15, EC 6.3.1.2, GLNSPIG59, Glutamate decarboxylase, glutamate-ammonia ligase, Glutamate--ammonia ligase, glutamate-ammonia ligase (glutamine synthase), glutamine synthase, glutamine synthetase, GS, PIG43, proliferation-inducing protein 43

Gene Symbol

GLUL

Additional Glutamine Synthetase Products

Product Documents for Glutamine Synthetase Antibody (8D7) - BSA Free

Certificate of Analysis

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Product Specific Notices for Glutamine Synthetase Antibody (8D7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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