Glutamine Synthetase Antibody - BSA Free

Novus Biologicals | Catalog # NB110-41404

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Glutamine Synthetase Primary Antibodies »

Product Specifications

Immunogen

Recombinant Glutamine Synthetase.

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glutamine Synthetase Antibody - BSA Free

Western Blot: Glutamine Synthetase Antibody [NB110-41404]

Western Blot: Glutamine Synthetase Antibody [NB110-41404]

Western Blot: Glutamine Synthetase Antibody [NB110-41404] - Analysis of glutamine synthase. 40ug of lysates from mouse (Lanes M), rat (Lane R), pig (Lane P), bovine (Lane B), or human (Lane Hu) retina were probed. A 42 kDa band was identified in lysates from retinas of all species.
Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody [NB110-41404]

Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody [NB110-41404]

Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NB110-41404] - Immunofluorescence using NB110-41404. Submitted via verified customer review.
Immunohistochemistry: Glutamine Synthetase Antibody [NB110-41404]

Immunohistochemistry: Glutamine Synthetase Antibody [NB110-41404]

Immunohistochemistry: Glutamine Synthetase Antibody [NB110-41404] - Localization of glutamine synthase in the retina. Paraffin sections of mouse (A, B), rat (C, D, G-I), or human (E, F) retina fixed in 4% paraformaldehyde were reacted with anti-glutamine synthase (red fluorescence staining in B, D, G, I, and brown immuno-peroxidase reaction. Nuclei in IF experiments (A-D) were stained with DAPI (cyan), and with nuclear fast red in E and F. At low magnification, anti-glutamine synthase reacted with a single population of cells extending from the ganglion cell layer through the inner nuclear layer. No signal was detected in controls either pre-incubated with 100ug/ml of the immunizing peptide (A) or with pre-immune serum (C, E). This finding was confirmed by co-localization (indicated by yellow in I) of glutamine synthase (red in G) with antoher marker of glutamine synthase (green in H).

Applications for Glutamine Synthetase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500 - 1:1000

Immunohistochemistry

1:1000

Western Blot

1:1000
Application Notes
This Glutamine Synthetase antibody is useful for Immunohistochemistry and Western Blot.

Reviewed Applications

Read 1 review rated 4 using NB110-41404 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glutamine Synthetase

Glutamine synthetase (GLUL) is an enzyme responsible for the de novo synthesis of glutamine (from glutamate and ammonia, wherein glutamate act as nitrogen donor/acceptor) and for production of gamma-aminobutyric acid/GABA (4-aminobutanoate). GLUL is expressed in a wide variety of human cells, such as astrocytes (marker protein), skeletal cells, adipocytes, hepatocytes and sperm. GLUL activity in vertebrate brain is generally high as it protects this ammonia-sensitive organ from harmful effects of ammonia and is involved in recycling of neurotransmitter glutamate also. GLUL is essential in maintaining cellular homeostasis and besides its involvement in ammonia detoxification, GLUL participates in maintaining amino acid/energy balance, cellular redox control, nucleotide biosynthesis and neurotransmitters regulation. Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) which is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death.

Alternate Names

cell proliferation-inducing protein 59, EC 4.1.1.15, EC 6.3.1.2, GLNSPIG59, Glutamate decarboxylase, glutamate-ammonia ligase, Glutamate--ammonia ligase, glutamate-ammonia ligase (glutamine synthase), glutamine synthase, glutamine synthetase, GS, PIG43, proliferation-inducing protein 43

Entrez Gene IDs

2752 (Human); 24957 (Rat)

Gene Symbol

GLUL

Additional Glutamine Synthetase Products

Product Documents for Glutamine Synthetase Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Glutamine Synthetase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Glutamine Synthetase Antibody - BSA Free

Customer Reviews for Glutamine Synthetase Antibody - BSA Free (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Glutamine Synthetase Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Mouse dorsal root ganglia
    Species: Mouse
    Verified Customer | Posted 05/11/2016
    Glutamine Synthetase Antibody - BSA Free NB110-41404

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Glutamine Synthetase Antibody - BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: I'm looking for a primary antibody specific to the cytosolic isoform of glutamine synthetase.

    A:

    I see no evidence that glutamine synthetase has multiple isoforms. Please see this link to UniProt (https://www.uniprot.org/uniprotkb/P15104/entry) for more details. NB110-41404 should work just fine for you as the only known isoform appears to be cytoplasmic.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies