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GlutaminePlus, powder Summary
Why Use GlutaminePlus in Cell Culture?
L-glutamine is an essential amino acid required as an energy and carbon source by virtually all cells in culture. Exhaustion of L-glutamine in cell culture media has been shown to cause both reduced cell viability and productivity. Supplementation of media with L-glutamine products is frequently required to provide additional nutrients for optimal performance of cells in culture. GlutaminePlus is a stable L-glutamine product that offers a more consistent delivery of L-glutamine to cells in culture as compared to L-glutamine.
How is L-Glutamine Utilized for Cell Culture?
Normal L-glutamine has a relatively short half-life in solution, showing a temperature-dependent loss of product with time. L-glutamine degrades through an intramolecular cyclization reaction forming, as by-products, pyrrolidone carbonic acid and ammonia in equimolar concentrations. The rate of breakdown increases with increasing temperature and in the presence of phosphate or bicarbonate. Dipeptide derivatives of L-glutamine prevent the intramolecular cyclization reaction associated with solutions of L-glutamine. These derivatives are therefore stable in solution and allow the formulation of cell culture media containing L-glutamine that may be stored at 4°C for extended periods. Solutions containing these derivatives can even be autoclaved without appreciable degradation of the product (30 minutes at 121°C results in approximately <5% loss of the product). The dipeptide derivatives are metabolized within the cells to yield L-glutamine plus the second amino acid. This results in more consistent delivery of L-glutamine to cells in culture and avoids toxic buildup of ammonia in cell cultures. This feature can be especially important for ammonia sensitive cell lines.
For research use only. Not for diagnostic use.
Are your L-Glutamine or GlutaminePlus formulated as powders sterile?
The powders are not provided sterile, we recommend filter sterilizing prepared solutions prior to use.
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