Glutathione Peroxidase 4/GPX4 Antibody

Novus Biologicals | Catalog # NBP2-76933

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Glutathione Peroxidase 4/GPX4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human Glutathione Peroxidase 4/GPX4 aa 1-197 / 197. (SwissProt: P36969 Human; SwissProt: O70325 Mouse; SwissProt: P36970 Rat)

Localization

Cytoplasm. Mitochondrion.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutathione Peroxidase 4/GPX4 Antibody

Western Blot: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933]

Western Blot: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933]

Western Blot: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - Western blot analysis of GPX4 on rat testis tissue lysate using anti- GPX4 antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933]

Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933]

Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - ICC staining GPX4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933]

Immunohistochemistry: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933]

Immunohistochemistry: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- GPX4 antibody. Counter stained with hematoxylin.
Flow Cytometry: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933]

Flow Cytometry: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933]

Flow Cytometry: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - Flow cytometric analysis of HepG2 cells with GPX4 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933]

Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933]

Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - ICC staining GPX4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933]

Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933]

Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - ICC staining GPX4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications for Glutathione Peroxidase 4/GPX4 Antibody

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry-Paraffin

1:50 -1:200

Western Blot

1:500-1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH7.4), 0.2% BSA, 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glutathione Peroxidase 4/GPX4

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.

Alternate Names

GPX4, PHGPx, snGPx

Gene Symbol

GPX4

Additional Glutathione Peroxidase 4/GPX4 Products

Product Documents for Glutathione Peroxidase 4/GPX4 Antibody

Certificate of Analysis

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Product Specific Notices for Glutathione Peroxidase 4/GPX4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Oxidative Stress
Redox Enzymes

Citations for Glutathione Peroxidase 4/GPX4 Antibody

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Protocols

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