Glutathione S Transferase kappa 1 Antibody

Novus Biologicals | Catalog # NBP2-16685

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Rhesus Macaque (92%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glutathione S Transferase kappa 1. The exact sequence is proprietary.

Reactivity Notes

Pig (83%), Bovine (80%)

Localization

Peroxisome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutathione S Transferase kappa 1 Antibody

Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody [NBP2-16685]

Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody [NBP2-16685]

Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody [NBP2-16685] - Analysis of Cal27 xenograft, using antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min.
Western Blot: Glutathione S Transferase kappa 1 Antibody [NBP2-16685]

Western Blot: Glutathione S Transferase kappa 1 Antibody [NBP2-16685]

Western Blot: Glutathione S Transferase kappa 1 Antibody [NBP2-16685] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 12% SDS PAGE gel, diluted at 1:1000.
Glutathione S Transferase kappa 1 Antibody

Western Blot: Glutathione S Transferase kappa 1 Antibody [NBP2-16685] -

Wild-type (WT) and Glutathione S-transferase kappa 1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glutathione S-transferase kappa 1 antibody (NBP2-16685) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Glutathione S Transferase kappa 1 Antibody

Immunocytochemistry/ Immunofluorescence: Glutathione S Transferase kappa 1 Antibody [NBP2-16685] -

Glutathione S-transferase kappa 1 antibody detects GSTK1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GSTK1 protein stained by Glutathione S-transferase kappa 1 antibody (NBP2-16685) diluted at 1:500.
Blue: Hoechst 33343 staining.

Applications for Glutathione S Transferase kappa 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glutathione S Transferase kappa 1

This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.

Alternate Names

EC 2.5.1.18, glutathione S-transferase k1, glutathione S-transferase kappa 1, Glutathione S-transferase subunit 13, glutathione S-transferase subunit 13 homolog, GST, GST 13-13, GST class-kappa, GST13, GST13-13, GSTK1-1, hGSTK1

Gene Symbol

GSTK1

UniProt

Additional Glutathione S Transferase kappa 1 Products

Product Documents for Glutathione S Transferase kappa 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Glutathione S Transferase kappa 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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