Glutathione S-Transferase pi 1/GSTP1 Antibody

Novus Biologicals | Catalog # NB100-1792

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Glutathione S-Transferase pi 1/GSTP1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-LADQGQSWKEEV corresponding to internal region according to NP_000843.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Glutathione S-Transferase pi 1/GSTP1 Antibody

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792]

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792]

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792] - (0.01ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792]

Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792]

Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792] - (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792]

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792]

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792] - Staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792]

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792]

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792] - HEK293 overexpressing GSTP1 and probed with (mock transfection in first lane).
Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792]

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792]

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792] - (RC203086) and probed with EB06802 (mock transfection in first lane), tested by Origene.

Applications for Glutathione S-Transferase pi 1/GSTP1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1:1000
Application Notes
WB: Approx. 24 kDa band observed in human kidney, liver and lung lysates (calculated MW of 23.4 kDa band according to NP_000843.1). In transfected HEK293 transiently expressing GSTP1 band of approx. 26 kDa band is observed. This band is not observed in the non-transfected HEK293.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glutathione S-Transferase pi 1/GSTP1

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.

Alternate Names

DFN7, FAEES3, Glutathione STransferase pi 1, GSTP1

Entrez Gene IDs

2950 (Human)

Gene Symbol

GSTP1

UniProt

P09211 (Human)

Additional Glutathione S-Transferase pi 1/GSTP1 Products

Product Documents for Glutathione S-Transferase pi 1/GSTP1 Antibody

Certificate of Analysis

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Product Specific Notices for Glutathione S-Transferase pi 1/GSTP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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