Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16756

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Predicted:

Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Glutathione S-Transferase pi 1/GSTP1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glutathione S-Transferase pi 1/GSTP1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (86%), Bovine (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756]

Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756]

Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756] - U87 xenograft, using GSTP1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GSTP1 antibody (NBP2-16756) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756] -

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
NBP2-16756 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756] -

GSTP1 antibody detects GSTP1 protein at nucleus on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
GSTP1 antibody (NBP2-16756) dilution: 1:500.
br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756] -

GSTP1 antibody detects GSTP1 protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
GSTP1 antibody (NBP2-16756) dilution: 1:500.

Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756] -

GSTP1 antibody detects GSTP1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
GSTP1 antibody (NBP2-16756) dilution: 1:500.
br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756] -

GSTP1 antibody detects GSTP1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSTP1 protein stained by GSTP1 antibody (NBP2-16756) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Hoechst 33342 staining.
Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756] -

GSTP1 antibody detects GSTP1 protein at nucleus on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
GSTP1 antibody (NBP2-16756) dilution: 1:500.

Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756] -

GSTP1 antibody detects GSTP1 protein by western blot analysis.
A. 50 ug Rat brain lysate/extract
12% SDS-PAGE
GSTP1 antibody (NBP2-16756) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756] -

GSTP1 antibody detects GSTP1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GSTP1 stained by GSTP1 antibody (NBP2-16756) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Reviewed Applications

Read 1 review rated 5 using NBP2-16756 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glutathione S-Transferase pi 1/GSTP1

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq]

Alternate Names

DFN7, FAEES3, Glutathione STransferase pi 1, GSTP1

Gene Symbol

GSTP1

UniProt

P09211 (Human)

Additional Glutathione S-Transferase pi 1/GSTP1 Products

Product Documents for Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Customer Reviews for Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Glutathione S-Transferase pi 1/GSTP1 Antibody
    Name: Hua Jiang
    Application: Western Blot
    Sample Tested: Liver, Mouse kidney, Adult lung and Mouse brain
    Species: Mouse and Human
    Verified Customer | Posted 11/18/2016
    Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free NBP2-16756

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies