Glycine Receptor Alpha 1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NB300-113

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all GLRA1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # P07727

Localization

Cell membrane

Specificity

Specific for the ~48 kDa alpha1- and alpha2-subunits of the glycine receptor in Western blots of rat spinal cord and brain stem and in cell extracts. Does not recognize other glycine receptor subunits.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for Glycine Receptor Alpha 1 Antibody - Azide and BSA Free

Western Blot: Glycine Receptor Alpha 1 AntibodyAzide and BSA Free [NB300-113]

Western Blot: Glycine Receptor Alpha 1 AntibodyAzide and BSA Free [NB300-113]

Western Blot: Glycine Receptor Alpha 1 Antibody [NB300-113] - Rat spinal cord lysate (SC) showing specific immunolabeling of the ~48 kDa alpha 1- and alpha 2-subunits of the glycine receptor. Immunolabeling was absent from a rat hippocampal lysate (H), as the glycine receptor is not expressed in the hippocampus.
Western Blot: Glycine Receptor Alpha 1 AntibodyAzide and BSA Free [NB300-113]

Western Blot: Glycine Receptor Alpha 1 AntibodyAzide and BSA Free [NB300-113]

Western Blot: Glycine Receptor Alpha 1 Antibody [NB300-113] - Rat spinal cord showing specific immunolabeling of the ~48k a1- and a2-subunits of the glycine receptor. The labeling was absent from a Rat hippocampal (hipp) lysate as the glycine receptor is not expressed in the hippocampus.
Western Blot: Glycine Receptor Alpha 1 AntibodyAzide and BSA Free [NB300-113]

Western Blot: Glycine Receptor Alpha 1 AntibodyAzide and BSA Free [NB300-113]

Western Blot: Glycine Receptor Alpha 1 Antibody [NB300-113] - Western blot of 10 ug of rat spinal cord. The antibody is specific for the ~48 kDa a1- and a2-subunitsof the glycine receptor.

Applications for Glycine Receptor Alpha 1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:1000

Western Blot

1:1000
Application Notes
Use in immunoprecipitation reported in scientific literature (PMID:31757808).

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute with 50 uL PBS to desired concentration.

Formulation

Lyophilized from 5 mM ammonium biocarbonate.

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GLRA1

Glycine is an important inhibitory neurotransmitter in the mammalian central nervous system, especially in the brainstem and spinal cord. It acts by binding to anion-conducting glycine receptors that belong to a superfamily of ligand-gated ion channels. Glycine receptors were first purified as strychnine binding sites in membrane fractions of adult spinal cord from rats (1). These strychnine-sensitive binding sites are different from the strychnine-insensitive binding sites found in the N-methyl-D-aspartate (NMDA) subtype of glutamate receptors. Adult glycine receptors are heterodimers composed of two subunits of 48 kDa and 58 kDa, denoted as a1 and b subunits. Fetal glycine receptors are homomers composed of a2 subunits.

Long Name

Glycine Receptor alpha 1

Alternate Names

HKPX1

Entrez Gene IDs

2741 (Human); 14654 (Mouse); 25674 (Rat)

Gene Symbol

GLRA1

UniProt

P07727 (Rat)

Additional GLRA1 Products

Product Documents for Glycine Receptor Alpha 1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Glycine Receptor Alpha 1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Glycine Receptor Alpha 1 Antibody - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Glycine Receptor Alpha 1 Antibody - Azide and BSA Free

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  • Q: Can you please let me know if you have antibodies against the following? For nAChR alpha 7 receptor of human origin - between amino acids 321- 465 For Glycine alpha1 receptor of human origin - between amino acids 339-413 For 5-HT 3A receptor of mouse origin - between amino acids 338-452

    A: We do not routinely epitope map our antibodies, and do not typically know where the antigen/antibody cites are. On occasion, customers will provide feedback about the epitopes, but this is not the norm. Unfortunately, we do not have any antibodies for nAChR alpha 7 receptor. For alpha 1 Glycine Receptor, we have two antibodies that target the N-terminus, NB300-113 and NBP1-49174, but I do not have more specific information about the epitope. For 5HT3A, we do have one antibody that may suit your needs, NB100-56351.

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