Glycine Receptor Alpha 1 Antibody

Novus Biologicals | Catalog # NBP2-26171

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Human (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GLRA1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence CLQAKDGISVKGAN corresponding to internal region according to NP_037265.1.

Specificity

The immunizing peptide represents part of the cytoplasmic domain. The antibody is not expected to cross-react with other glycine receptor alpha proteins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Glycine Receptor Alpha 1 Antibody

Western Blot: Glycine Receptor Alpha 1 Antibody [NBP2-26171]

Western Blot: Glycine Receptor Alpha 1 Antibody [NBP2-26171]

Western Blot: Glycine Receptor Alpha 1 Antibody [NBP2-26171] - Staining of Mouse Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Glycine Receptor Alpha 1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in mouse and rat testis lysates (calculated MW of 51.7 kDa band according to mouse NP_065238.2). The observed molecular weight corresponds to the glycosylated form.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GLRA1

Glycine is an important inhibitory neurotransmitter in the mammalian central nervous system, especially in the brainstem and spinal cord. It acts by binding to anion-conducting glycine receptors that belong to a superfamily of ligand-gated ion channels. Glycine receptors were first purified as strychnine binding sites in membrane fractions of adult spinal cord from rats (1). These strychnine-sensitive binding sites are different from the strychnine-insensitive binding sites found in the N-methyl-D-aspartate (NMDA) subtype of glutamate receptors. Adult glycine receptors are heterodimers composed of two subunits of 48 kDa and 58 kDa, denoted as a1 and b subunits. Fetal glycine receptors are homomers composed of a2 subunits.

Long Name

Glycine Receptor alpha 1

Alternate Names

HKPX1

Entrez Gene IDs

2741 (Human); 14654 (Mouse); 25674 (Rat)

Gene Symbol

GLRA1

Additional GLRA1 Products

Product Documents for Glycine Receptor Alpha 1 Antibody

Certificate of Analysis

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Product Specific Notices for Glycine Receptor Alpha 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Glycine Receptor Alpha 1 Antibody

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Protocols

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