Glycogen phosphorylase, muscle form Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16689

Novus Biologicals
Loading...

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Cited:

Rat, green monkey

Predicted:

Bovine (96%), Rabbit (95%), Rhesus Macaque (98%), Sheep (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
Loading...

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glycogen phosphorylase, muscle form. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (84%). Rat reactivity reported in scientific literature (PMID: 30660767).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glycogen phosphorylase, muscle form Antibody - BSA Free

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689]

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689]

Glycogen phosphorylase- muscle form Antibody-Western Blot-NBP2-16689-img0008.jpg
Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689]

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689]

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000
Glycogen phosphorylase, muscle form Antibody

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PYGM antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Glycogen phosphorylase, muscle form Antibody

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PYGM antibody [C1C3] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Glycogen phosphorylase, muscle form Antibody

Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -

Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Mouse muscle. PYGM stained by PYGM antibody [C1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Glycogen phosphorylase, muscle form Antibody

Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -

Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -Analysis of paraffin-embedded zebrafish tissue, using PYGM antibody [C1C3] at 1:300 dilution.
Glycogen phosphorylase, muscle form Antibody

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Region-based patterns of glycogen phosphorylase-muscle type (GPmm) Protein expression in icv Lz-pretreated male & female rats. GPmm protein was measured by Western blot in the rostral (a; F(7,40) = 35.87; p < 0.0001), middle (b; F(7,40) = 33.89; p < 0.0001), & caudal (c; F(7,40) = 9.43; p < 0.0001) VMN of V/V, V/INS, Lz/V, & Lz/INS groups of male (M; left-hand side) & female (F; right-hand side) rats. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each VMN segment, data depict mean normalized GAD O.D. values ± S.E.M. Results are summarized in the table at bottom; ↑ & ↓ denote a relative increase or decrease, respectively, between treatment groups; no change (NC) between groups is also indicated Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33238883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Glycogen phosphorylase, muscle form Antibody

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Region-based patterns of glycogen phosphorylase-muscle type (GPmm) Protein expression in icv Lz-pretreated male & female rats. GPmm protein was measured by Western blot in the rostral (a; F(7,40) = 35.87; p < 0.0001), middle (b; F(7,40) = 33.89; p < 0.0001), & caudal (c; F(7,40) = 9.43; p < 0.0001) VMN of V/V, V/INS, Lz/V, & Lz/INS groups of male (M; left-hand side) & female (F; right-hand side) rats. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each VMN segment, data depict mean normalized GAD O.D. values ± S.E.M. Results are summarized in the table at bottom; ↑ & ↓ denote a relative increase or decrease, respectively, between treatment groups; no change (NC) between groups is also indicated Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33238883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Glycogen phosphorylase, muscle form Antibody

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - ER alpha & ER beta Involvement in Noradrenergic Regulation of VMN GS & GPbb/GPmm Protein Expression. Micropunch-dissected VMN tissue obtained from groups of female rats (n = 6/group) infused into the VMN with Veh or NE after Veh, MPP, or PHTPP pretreatment was analyzed by Western blot for GS (Panel 5A), F(5, 12) = 8.44, p = .0003; GPbb (Panel 5B), F(5, 12) = 12.90, p < .0001; or GPmm (Panel 5C), F(5, 12) = 16.49, p < .0001 protein content. Data show mean normalized protein optical density (O.D.) values ± SEM. *p < .05; **p < .01; ***p < .001. VMN = ventromedial hypothalamic nucleus; GS = glycogen synthase; GPmm = glycogen phosphorylase-muscle type; GPbb = glycogen phosphorylase-brain type; MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Glycogen phosphorylase, muscle form Antibody - BSA Free

Immunohistochemistry: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -

PYGM antibody [C1C3] detects PYGM protein at cytoplasm on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
PYGM antibody [C1C3] (NBP2-16689) dilution: 1:500.
br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

Applications for Glycogen phosphorylase, muscle form Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glycogen phosphorylase, muscle form

Glycogen phosphorylase (EC 2.4.1.1) catalyzes and regulates the breakdown of glycogen to glucose-1-phosphate.[supplied by OMIM]

Long Name

Glycogen phosphorylase, muscle form

Alternate Names

EC 2.4.1.1, Myophosphorylase, PYGM

Gene Symbol

PYGM

UniProt

Additional Glycogen phosphorylase, muscle form Products

Product Documents for Glycogen phosphorylase, muscle form Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Glycogen phosphorylase, muscle form Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Glycogen phosphorylase, muscle form Antibody - BSA Free

Customer Reviews for Glycogen phosphorylase, muscle form Antibody - BSA Free

There are currently no reviews for this product. Be the first to review Glycogen phosphorylase, muscle form Antibody - BSA Free and earn rewards!

Have you used Glycogen phosphorylase, muscle form Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies
Loading...