Glycogen phosphorylase, muscle form Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-16689
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Zebrafish
Cited:
Rat, green monkey
Predicted:
Bovine (96%), Rabbit (95%), Rhesus Macaque (98%), Sheep (96%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Glycogen phosphorylase, muscle form. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (84%). Rat reactivity reported in scientific literature (PMID: 30660767).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Glycogen phosphorylase, muscle form Antibody - BSA Free
Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689]
Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -
Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PYGM antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -
Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PYGM antibody [C1C3] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -
Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Mouse muscle. PYGM stained by PYGM antibody [C1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -
Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -Analysis of paraffin-embedded zebrafish tissue, using PYGM antibody [C1C3] at 1:300 dilution.Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -
Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Region-based patterns of glycogen phosphorylase-muscle type (GPmm) Protein expression in icv Lz-pretreated male & female rats. GPmm protein was measured by Western blot in the rostral (a; F(7,40) = 35.87; p < 0.0001), middle (b; F(7,40) = 33.89; p < 0.0001), & caudal (c; F(7,40) = 9.43; p < 0.0001) VMN of V/V, V/INS, Lz/V, & Lz/INS groups of male (M; left-hand side) & female (F; right-hand side) rats. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each VMN segment, data depict mean normalized GAD O.D. values ± S.E.M. Results are summarized in the table at bottom; ↑ & ↓ denote a relative increase or decrease, respectively, between treatment groups; no change (NC) between groups is also indicated Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33238883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -
Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Region-based patterns of glycogen phosphorylase-muscle type (GPmm) Protein expression in icv Lz-pretreated male & female rats. GPmm protein was measured by Western blot in the rostral (a; F(7,40) = 35.87; p < 0.0001), middle (b; F(7,40) = 33.89; p < 0.0001), & caudal (c; F(7,40) = 9.43; p < 0.0001) VMN of V/V, V/INS, Lz/V, & Lz/INS groups of male (M; left-hand side) & female (F; right-hand side) rats. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each VMN segment, data depict mean normalized GAD O.D. values ± S.E.M. Results are summarized in the table at bottom; ↑ & ↓ denote a relative increase or decrease, respectively, between treatment groups; no change (NC) between groups is also indicated Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33238883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -
Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - ER alpha & ER beta Involvement in Noradrenergic Regulation of VMN GS & GPbb/GPmm Protein Expression. Micropunch-dissected VMN tissue obtained from groups of female rats (n = 6/group) infused into the VMN with Veh or NE after Veh, MPP, or PHTPP pretreatment was analyzed by Western blot for GS (Panel 5A), F(5, 12) = 8.44, p = .0003; GPbb (Panel 5B), F(5, 12) = 12.90, p < .0001; or GPmm (Panel 5C), F(5, 12) = 16.49, p < .0001 protein content. Data show mean normalized protein optical density (O.D.) values ± SEM. *p < .05; **p < .01; ***p < .001. VMN = ventromedial hypothalamic nucleus; GS = glycogen synthase; GPmm = glycogen phosphorylase-muscle type; GPbb = glycogen phosphorylase-brain type; MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunohistochemistry: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -
PYGM antibody [C1C3] detects PYGM protein at cytoplasm on human breast cancer by immunohistochemical analysis.Sample: Paraffin-embedded breast cancer.
PYGM antibody [C1C3] (NBP2-16689) dilution: 1:500.
br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
Applications for Glycogen phosphorylase, muscle form Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Glycogen phosphorylase, muscle form
Long Name
Glycogen phosphorylase, muscle form
Alternate Names
EC 2.4.1.1, Myophosphorylase, PYGM
Gene Symbol
PYGM
UniProt
Additional Glycogen phosphorylase, muscle form Products
Product Documents for Glycogen phosphorylase, muscle form Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Glycogen phosphorylase, muscle form Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Citations for Glycogen phosphorylase, muscle form Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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