Glycogen Synthase [p Ser641] Antibody (SR46-06)

Western Blot: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of Phospho-Glycogen synthase 1 on HepG2 cell lysates. Lane 1: HepG2 cells, whole cell lysate, 10ug/lane Lane 2: HepG2 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Glycogen synthase 1 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 84 kDa Observed band size: 84 kDa Blocking and diluting buffer: 5% BSA.Exposure time: 1 minute 34 seconds

Immunocytochemistry/Immunofluorescence: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Staining Phospho-Glycogen synthase 1(S641) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Glycogen synthase [p Ser641] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.