Glyoxalase I Antibody

Novus Biologicals | Catalog # NBP1-31466

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (90%), Rat (91%), Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Glyoxalase I Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glyoxalase I. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (82%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glyoxalase I Antibody

Western Blot: Glyoxalase I Antibody [NBP1-31466]

Western Blot: Glyoxalase I Antibody [NBP1-31466]

Western Blot: Glyoxalase I Antibody [NBP1-31466] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/ Immunofluorescence: Glyoxalase I Antibody [NBP1-31466]

Immunocytochemistry/ Immunofluorescence: Glyoxalase I Antibody [NBP1-31466]

Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody [NBP1-31466] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glyoxalase I protein stained by Glyoxalase I antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: Glyoxalase I Antibody [NBP1-31466]

Immunohistochemistry-Paraffin: Glyoxalase I Antibody [NBP1-31466]

Immunohistochemistry-Paraffin: Glyoxalase I Antibody [NBP1-31466] - Human ovarian cancer. Glyoxalase I stained by Glyoxalase I antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Glyoxalase I Antibody [NBP1-31466]

Western Blot: Glyoxalase I Antibody [NBP1-31466]

Western Blot: Glyoxalase I Antibody [NBP1-31466] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.
Glyoxalase I Antibody

Western Blot: Glyoxalase I Antibody [NBP1-31466] -

Western Blot: Glyoxalase I Antibody [NBP1-31466] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Glyoxalase I Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP1-31466 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glyoxalase I

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.

Alternate Names

Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, Methylglyoxalase

Entrez Gene IDs

2739 (Human)

Gene Symbol

GLO1

UniProt

Q04760 (Human)

Additional Glyoxalase I Products

Product Documents for Glyoxalase I Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Glyoxalase I Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Glyoxalase I Antibody

Customer Reviews for Glyoxalase I Antibody (1)

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  • Glyoxalase I Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse heart
    Species: Mouse
    Verified Customer | Posted 03/18/2019
    Tissues were collected after myocardial infarction. For western blot, 20 ug/mL total protein were loaded in each well.
    Glyoxalase I Antibody NBP1-31466

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