Glypican 1 Antibody

Novus Biologicals | Catalog # NBP1-33197

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Glypican 1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (89%).

Localization

Cell membrane, Lipid-anchor, GPI-anchor, Extracellular side, Secreted glypican-1: Secreted, extracellular space

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glypican 1 Antibody

Western Blot: Glypican 1 Antibody [NBP1-33197]

Western Blot: Glypican 1 Antibody [NBP1-33197]

Western Blot: Glypican 1 Antibody [NBP1-33197] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3].The observed M.W. is based on the publication: PMID: 22351761, 26203194 and 21932778
Western Blot: Glypican 1 Antibody [NBP1-33197]

Western Blot: Glypican 1 Antibody [NBP1-33197]

Western Blot: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3].The observed M.W. is based on the following publications: PMID: 22351761, 26203194 and 21932778
Immunocytochemistry/ Immunofluorescence: Glypican 1 Antibody [NBP1-33197]

Immunocytochemistry/ Immunofluorescence: Glypican 1 Antibody [NBP1-33197]

Immunocytochemistry/Immunofluorescence: Glypican 1 Antibody [NBP1-33197] - Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: Glypican 1 stained by Glypican 1 antibody [N3C3] diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 um.
Immunohistochemistry-Paraffin: Glypican 1 Antibody [NBP1-33197]

Immunohistochemistry-Paraffin: Glypican 1 Antibody [NBP1-33197]

Immunohistochemistry-Paraffin: Glypican 1 Antibody [NBP1-33197] - Paraffin-embedded human normal pancreas (left) and pancreatic cancer (right).Glypican 1 stained by Glypican 1 antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Flow Cytometry: Glypican 1 Antibody [NBP1-33197]

Flow Cytometry: Glypican 1 Antibody [NBP1-33197]

Flow Cytometry: Glypican 1 Antibody [NBP1-33197] - Sample: A431 cell. Black: Unlabelled sample was used as a control. Red: Glypican 1 antibody [N3C3]dilution: 1:100. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
Western Blot: Glypican 1 Antibody [NBP1-33197]

Western Blot: Glypican 1 Antibody [NBP1-33197]

Western Blot: Glypican 1 Antibody [NBP1-33197] - Mouse tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500.
Western Blot: Glypican 1 Antibody [NBP1-33197]

Western Blot: Glypican 1 Antibody [NBP1-33197]

Western Blot: Glypican 1 Antibody [NBP1-33197] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody ] was used to detect the primary antibody.
Western Blot: Glypican 1 Antibody [NBP1-33197]

Western Blot: Glypican 1 Antibody [NBP1-33197]

Western Blot: Glypican 1 Antibody [NBP1-33197] - U87-MG whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Glypican 1 Antibody [NBP1-33197]

Western Blot: Glypican 1 Antibody [NBP1-33197]

Western Blot: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
The observed M.W. is based on the following publications: PMID: 22351761, 26203194 and 21932778
ELISA: Glypican 1 Antibody [NBP1-33197]

ELISA: Glypican 1 Antibody [NBP1-33197]

ELISA: Glypican 1 Antibody [NBP1-33197] - An ELISA plate is coated with MCF-7 and A431 cells. The coated cells are detected with Glypican 1 antibody at concentration ranged from 7.5 to 240 ng/mL.
Immunoprecipitation: Glypican 1 Antibody [NBP1-33197]

Immunoprecipitation: Glypican 1 Antibody [NBP1-33197]

Immunoprecipitation: Glypican 1 Antibody [NBP1-33197] - Immunoprecipitation of Glypican 1 protein from MCF-7 whole cell extracts using 5 ug of Glypican 1 antibody [N3C3].
Glypican 1 Antibody

Immunohistochemistry-Paraffin: Glypican 1 Antibody [NBP1-33197] -

Immunohistochemistry-Paraffin: Glypican 1 Antibody [NBP1-33197] - Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
Glypican 1 stained by Glypican 1 antibody [N3C3] (NBP1-33197) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Glypican 1 Antibody

Western Blot: Glypican 1 Antibody [NBP1-33197] -

Western Blot: Glypican 1 Antibody [NBP1-33197] - A431 whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (NBP1-33197) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Glypican 1 Antibody

Western Blot: Glypican 1 Antibody [NBP1-33197] -

Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (NBP1-33197) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Glypican 1 Antibody

Western Blot: Glypican 1 Antibody [NBP1-33197] -

A431 whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (NBP1-33197) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Glypican 1 Antibody

Application
Recommended Usage

ELISA

1:1000-1:10000

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glypican 1

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Alternate Names

GPC1

Gene Symbol

GPC1

Additional Glypican 1 Products

Product Documents for Glypican 1 Antibody

Certificate of Analysis

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Product Specific Notices for Glypican 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Proteoglycans
Wnt Signaling Modulators

Citations for Glypican 1 Antibody

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Protocols

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