Glypican 4 Antibody (51E3) - BSA Free

Novus Biologicals | Catalog # NBP2-49892

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 51E3

Format

BSA Free
View all Glypican 4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Glypican 4 (401-529aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Glypican 4 Antibody (51E3) - BSA Free

Western Blot: Glypican 4 Antibody (51E3)BSA Free [NBP2-49892]

Western Blot: Glypican 4 Antibody (51E3)BSA Free [NBP2-49892]

Western Blot: Glypican 4 Antibody (51E3) [NBP2-49892] - Glypican 4 Antibody (AT51E3) [NBP2-49892] - The Recombinant Human GPC4 (50ng) and Cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPC4 anibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human GPC4 Lane 2. : Ramos cell lysate
Immunocytochemistry/ Immunofluorescence: Glypican 4 Antibody (51E3) - BSA Free [NBP2-49892]

Immunocytochemistry/ Immunofluorescence: Glypican 4 Antibody (51E3) - BSA Free [NBP2-49892]

Immunocytochemistry/Immunofluorescence: Glypican 4 Antibody (51E3) [NBP2-49892] - Glypican 4 Antibody (AT51E3) [NBP2-49892] - analysis of GPC4 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GPC4 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: Glypican 4 Antibody (51E3) - BSA Free [NBP2-49892]

Flow Cytometry: Glypican 4 Antibody (51E3) - BSA Free [NBP2-49892]

Flow Cytometry: Glypican 4 Antibody (51E3) [NBP2-49892] - Analysis of GPC4 in 293T cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Applications for Glypican 4 Antibody (51E3) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:200

Immunocytochemistry/ Immunofluorescence

1:200

Western Blot

1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glypican 4

The glypican (Gpc) family of cell surface heparan sulfate proteoglycans is expressed in a tissue specific and developmentally regulated fashion. Expression of glypican 4 has been reported in the mouse brain at embryonic day 10 and later stages and low during early tubule formation but is up-regulated in mature tubules. Accordingly it is not detected in the midline of the embryo at the stage of intiation of neural tube closure, suggesting that glypican-4 is unlikely to play an essential role in convergent extension in the mouse

Alternate Names

GPC4, K-glypican

Gene Symbol

GPC4

Additional Glypican 4 Products

Product Documents for Glypican 4 Antibody (51E3) - BSA Free

Certificate of Analysis

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Product Specific Notices for Glypican 4 Antibody (51E3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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