GM130/GOLGA2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-53420

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GM130/GOLGA2 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant human GM130/GOLGA2 protein (amino acids 528-606). [UniPro Q08379]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GM130/GOLGA2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP2-53420 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-GM130/GOLGA2 Antibody NBP2-53420 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-GM130/GOLGA2 Antibody NBP2-53420 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - HeLa cells were fixed for 10 min using 10% formalin and then permeabilized for 5 min using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-GOLGA2 at 1:200 overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: GM130/GOLGA2 AntibodyBSA Free [NBP2-53420]

Western Blot: GM130/GOLGA2 AntibodyBSA Free [NBP2-53420]

Western Blot: GM130/GOLGA2 Antibody [NBP2-53420] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-GOLGA2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP2-53420] - Analysis of a FFPE tissue section of mouse intesting using GM130/GOLGA2 antibody at 1:1500. The antibody generated staining in the the Golgi apparatus in the perinuclear region.
Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with GM130/GOLGA2 Antibody conjugated to Biotin (NBP2-53420B) at 5 ug/ml for 1 hour at room temperature and detected with Streptavidin conjugated to DyLight 488. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with GM130/GOLGA2 Antibody conjugated to FITC (NBP2-53420F) at 2 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Flow Cytometry: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Flow Cytometry: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Flow Cytometry: GM130/GOLGA2 Antibody [NBP2-53420] - An intracellular stain was performed on NIH3T3 cells with NBP2-53420 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with GM130/GOLGA2 Antibody conjugated to FITC (NBP2-53420F) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Flow Cytometry: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Flow Cytometry: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Flow Cytometry: GM130/GOLGA2 Antibody [NBP2-53420] - An intracellular stain was performed on HeLa cells with NBP2-53420AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 min at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Flow Cytometry: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Flow Cytometry: GM130/GOLGA2 Antibody [NBP2-53420] - An intracellular stain was performed on MCF7 cells with GM130/GOLGA2 Antibody NBP2-53420AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Flow Cytometry: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420]

Flow Cytometry: GM130/GOLGA2 Antibody [NBP2-53420] - An intracellular stain was performed on HeLa cells with NBP2-53420 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
GM130/GOLGA2 Antibody - BSA Free

Immunohistochemistry: Rabbit Polyclonal GM130/GOLGA2 Antibody [NBP2-53420] -

Immunohistochemistry: Rabbit Polyclonal GM130/GOLGA2 Antibody [NBP2-53420] - Staining of mouse C57BL/6 retina using GM130/GOLGA2 Antibody. GOLGA (red), BS-1 lectin (green), DAPI (blue). Primary antibody dilution: 1:200. Image from a verified customer review.
GM130/GOLGA2 Antibody - BSA Free

Western Blot: GM130/GOLGA2 Antibody - BSA Free [NBP2-53420] -

Extracellular vesicle secretion induced by Sorafenib and their miRNA content. (A) Particle size and concentration analysis of Large, Small, and Very Small EVs obtained at 6 and 24 h. (B) Expression of EV markers and cellular contaminants in Large, Small, and Very Small EVs and cell lysates obtained at 24 h after Sorafenib treatment. Total lane protein content was used as the loading control. (C) Representative images of cryo-EM of Small and Very Small EVs obtained at 6 and 24 h from the control and Sorafenib-treated cells. (D) Assessment of EV size (nm) in the cryo-EM images. (E) Number of EVs quantified in the cryo-EM images. (F) miRNA expression in the three fractions of EVs at 6 h. (G) miRNA expression in the three fractions of EVs at 24 h. Fold-change values were calculated between Sorafenib and the control treated samples. Results are expressed as the mean +/- SEM of six independent experiments. Ns, non-significant; * p ≤0.05, ** p ≤ 0.01, *** p ≤ 0.001, and **** p ≤ 0.0001 between the miRNA expression in the control and Sorafenib derived EVs. Multiple comparison test statistics are expressed with lower case a–i. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36078082), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for GM130/GOLGA2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-5 ug/mL

Immunohistochemistry

1:1000 - 1:1500

Immunohistochemistry-Paraffin

1:1000 - 1:1500

Western Blot

0.5 ug/mL

Reviewed Applications

Read 1 review rated 5 using NBP2-53420 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GM130/GOLGA2

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. GM130 encodes a member of the Golgin protein family and is localised to the Golgi. The protein encoded by GM130 has been postulated to play roles in the stacking of Golgi cisternae, maintaining cis-Golgi structure and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. GM130 is part of a larger oligomeric complex which interacts with p115, GORASP1/GRASP65 and RAB1B that has been activated by GTP-binding. GM130 is useful as a cis-Golgi marker in immunohistochemistry.

Long Name

Golgin A2

Alternate Names

GOLGA2, Golgin-95, SY11 Protein

Gene Symbol

GOLGA2

Additional GM130/GOLGA2 Products

Product Documents for GM130/GOLGA2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GM130/GOLGA2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Inflammatory Mediators

Citations for GM130/GOLGA2 Antibody - BSA Free

Customer Reviews for GM130/GOLGA2 Antibody - BSA Free (1)

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Customer Images

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  • GM130/GOLGA2 Antibody - BSA Free
    Name: Anonymous
    Application: IF- fixed tissue
    Sample Tested: C57BL/6 mouse retina
    Species: Mouse
    Verified Customer | Posted 05/09/2024
    GOLGA (red), BS-1 lectin (green), DAPI (blue)
    1:200
    GM130/GOLGA2 Antibody - BSA Free NBP2-53420

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