GMDS Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33424

Novus Biologicals
Loading...

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (97%), Mouse (99%), Rat (98%), Rhesus Macaque (99%), Xenopus (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
Loading...

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GMDS. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (93%), Zebrafish (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GMDS Antibody - BSA Free

Knockdown Validated: GMDS Antibody [NBP1-33424]

Western Blot: GMDS Antibody [NBP1-33424]

GMDS-Antibody-Knockdown-Validated-NBP1-33424-img0008.jpg
Immunohistochemistry: GMDS Antibody [NBP1-33424]

Immunohistochemistry: GMDS Antibody [NBP1-33424]

GMDS-Antibody-Immunohistochemistry-NBP1-33424-img0006.jpg
Western Blot: GMDS Antibody [NBP1-33424]

Western Blot: GMDS Antibody [NBP1-33424]

GMDS-Antibody-Western-Blot-NBP1-33424-img0007.jpg
Western Blot: GMDS Antibody [NBP1-33424]

Western Blot: GMDS Antibody [NBP1-33424]

Western Blot: GMDS Antibody [NBP1-33424] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 10% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: GMDS Antibody [NBP1-33424]

Immunohistochemistry-Paraffin: GMDS Antibody [NBP1-33424]

Immunohistochemistry-Paraffin: GMDS Antibody [NBP1-33424] - DLD1 xenograft, using GMDS antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: GMDS Antibody [NBP1-33424]

Immunohistochemistry-Paraffin: GMDS Antibody [NBP1-33424]

Immunohistochemistry-Paraffin: GMDS Antibody [NBP1-33424] - Human breast carcinoma. GMDS antibody [N2C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
GMDS Antibody

Western Blot: GMDS Antibody [NBP1-33424] -

Western Blot: GMDS Antibody [NBP1-33424] - Impaired cell proliferation in human lung adenocarcinoma cell lines with GMDS knockdown via Cellomics ArrayScan VTI. a GMDS mRNA level in A549 cells & H1299 cells infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA examined by quantitative real-time PCR (normalized to GAPDH mRNA). Data shown here was one out of three independent experiments (**, p < 0.01). b Relative GMDS protein level in A549 cells & H1299 cells infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA examined by western blot. GAPDH protein was used as internal control. c-d. Representative microscope pictures of A549 cells (c) & H1299 cells (d) infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA at different time points. e-f. Proliferation profiling of A549 cells (e) & H1299 cells (f) infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA for continuous 5 days examined by Cellomics ArrayScan VTI. Histogram shown here was relative fold changes of cell numbers compared to Day 1 & representing the mean ± SEM of three independent experiments (**, p < 0.01) Image collected & cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4524-1), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for GMDS Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GMDS

GDP-mannose 4,6-dehydratase (GMD; EC 4.2.1.47) catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose,the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor. The second and third stepsof the pathway are catalyzed by a single enzyme, GDP-keto-6-deoxymannose 3,5-epimerase, 4-reductase, designated FX inhumans (MIM 137020).(supplied by OMIM)

Alternate Names

EC 4.2.1.47, GDP-D-mannose dehydratase, GDP-mannose 4,6-dehydratase, GMDGDP-mannose 4,6 dehydratase, SDR3E1, short chain dehydrogenase/reductase family 3E, member 1

Entrez Gene IDs

2762 (Human)

Gene Symbol

GMDS

UniProt

Additional GMDS Products

Product Documents for GMDS Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GMDS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for GMDS Antibody - BSA Free

Customer Reviews for GMDS Antibody - BSA Free

There are currently no reviews for this product. Be the first to review GMDS Antibody - BSA Free and earn rewards!

Have you used GMDS Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies
Loading...