GMDS Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-33424
Loading...
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Bovine (97%), Mouse (99%), Rat (98%), Rhesus Macaque (99%), Xenopus (93%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human GMDS. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (93%), Zebrafish (89%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GMDS Antibody - BSA Free
Immunohistochemistry: GMDS Antibody [NBP1-33424]
GMDS-Antibody-Immunohistochemistry-NBP1-33424-img0006.jpgWestern Blot: GMDS Antibody [NBP1-33424]
GMDS-Antibody-Western-Blot-NBP1-33424-img0007.jpgWestern Blot: GMDS Antibody [NBP1-33424]
Western Blot: GMDS Antibody [NBP1-33424] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 10% SDS PAGE; antibody diluted at 1:1000.Immunohistochemistry-Paraffin: GMDS Antibody [NBP1-33424]
Immunohistochemistry-Paraffin: GMDS Antibody [NBP1-33424] - DLD1 xenograft, using GMDS antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.Immunohistochemistry-Paraffin: GMDS Antibody [NBP1-33424]
Immunohistochemistry-Paraffin: GMDS Antibody [NBP1-33424] - Human breast carcinoma. GMDS antibody [N2C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.Western Blot: GMDS Antibody [NBP1-33424] -
Western Blot: GMDS Antibody [NBP1-33424] - Impaired cell proliferation in human lung adenocarcinoma cell lines with GMDS knockdown via Cellomics ArrayScan VTI. a GMDS mRNA level in A549 cells & H1299 cells infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA examined by quantitative real-time PCR (normalized to GAPDH mRNA). Data shown here was one out of three independent experiments (**, p < 0.01). b Relative GMDS protein level in A549 cells & H1299 cells infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA examined by western blot. GAPDH protein was used as internal control. c-d. Representative microscope pictures of A549 cells (c) & H1299 cells (d) infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA at different time points. e-f. Proliferation profiling of A549 cells (e) & H1299 cells (f) infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA for continuous 5 days examined by Cellomics ArrayScan VTI. Histogram shown here was relative fold changes of cell numbers compared to Day 1 & representing the mean ± SEM of three independent experiments (**, p < 0.01) Image collected & cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4524-1), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for GMDS Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: GMDS
Alternate Names
EC 4.2.1.47, GDP-D-mannose dehydratase, GDP-mannose 4,6-dehydratase, GMDGDP-mannose 4,6 dehydratase, SDR3E1, short chain dehydrogenase/reductase family 3E, member 1
Entrez Gene IDs
2762 (Human)
Gene Symbol
GMDS
UniProt
Additional GMDS Products
Product Documents for GMDS Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GMDS Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Citations for GMDS Antibody - BSA Free
Customer Reviews for GMDS Antibody - BSA Free
There are currently no reviews for this product. Be the first to review GMDS Antibody - BSA Free and earn rewards!
Have you used GMDS Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...