GMEB1 Antibody

Name: GMEB1 Antibody
Brand: Novus Biologicals
SKU: NBP1-46207
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-46207

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 523 and 573 of human Glucocorticoid Modulatory Element Binding Protein 1, using the numbering given in entry NP_006573.2 (GeneID 10691).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GMEB1 Antibody

Western Blot: GMEB1 Antibody [NBP1-46207]

Western Blot: GMEB1 Antibody [NBP1-46207] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-GMEB1 antibody NBP1-46207used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. GMEB1 was also immunoprecipitated by another rabbit anti-GMEB1 antibody, which recognizes an upstream epitope.

Immunohistochemistry-Paraffin: GMEB1 Antibody [NBP1-46207]

Immunohistochemistry-Paraffin: GMEB1 Antibody [NBP1-46207] - Sample: FFPE section of human lung carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-GMEB1 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Applications for GMEB1 Antibody

Application

Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Application Notes

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GMEB1

This gene is a member of KDWK gene family. The product of this gene associates with GMEB2 protein, and the complex is essential for parvovirus DNA replication. Study of rat homolog implicates the role of this gene in modulation of transactivation by the glucocorticoid receptor bound to glucocorticoid response elements. Two alternative spliced transcript variants encoding different isoforms exist.

Alternate Names

DNA-binding protein p96PIF, glucocorticoid modulatory element binding protein 1, glucocorticoid modulatory element-binding protein 1, GMEB-1, P96PIF, Parvovirus initiation factor p96, PIF p96, PIF96

Entrez Gene IDs

10691 (Human)

Gene Symbol

GMEB1

UniProt

Q9Y692 (Human)

Additional GMEB1 Products

Product Documents for GMEB1 Antibody

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Certificate of Analysis

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Product Specific Notices for GMEB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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