GNB3 Antibody

Name: GNB3 Antibody
Brand: Novus Biologicals
SKU: NBP1-52107
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-52107

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Peptide with sequence SGHDNRVSCLGVT corresponding to internal region according to NP_002066.1, NP_067642.1.

Epitope

SGHDNRVSCLGVT

Specificity

This antibody is expected to recognize GNB3 & GNB4 (GeneID: 2784, 59345; ProteinID: NP_002066.1; NP_067642.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GNB3 Antibody

Western Blot: GNB3 Antibody [NBP1-52107]

Western Blot: GNB3 Antibody [NBP1-52107] - Analysis of GNB3 in HepG2 lysate (35ug protein in RIPA buffer) using this antibody at 0.1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: GNB3 Antibody [NBP1-52107]

Immunohistochemistry-Paraffin: GNB3 Antibody [NBP1-52107] - (3.8ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for GNB3 Antibody

Application

Recommended Usage

Immunohistochemistry

3 - 5 ug/ml

Immunohistochemistry-Paraffin

3 - 5 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/ml

Application Notes

WB: Approx. 35 kDa band observed in HepG2 lysates (calculated MW of 37.2 kDa band according to NP_002066.1. IHC-P: Human cerebellum shows strong axonal staining in the molecular layer and less so in the granular layer.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GNB3

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. A single-nucleotide polymorphism (C825T) in this gene is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Additional splice variants may exist for this gene, but they have not been fully described.

Alternate Names

G protein, beta-3 subunit, GTP-binding regulatory protein beta-3 chain, guanine nucleotide binding protein (G protein), beta polypeptide 3, guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 3, guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3, hypertension associated protein, Transducin beta chain 3

Entrez Gene IDs

60449 (Rat)

Gene Symbol

GNB3

UniProt

NP_002066.1 (Human)

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Product Documents for GNB3 Antibody

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Product Specific Notices for GNB3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GNB3 Antibody

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