GNIP Antibody

Novus Biologicals | Catalog # NB100-1229

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GNIP Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QAPAGPPWDITEA corresponding to C-Terminus according to NP_203128.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GNIP Antibody

Western Blot: GNIP Antibody [NB100-1229]

Western Blot: GNIP Antibody [NB100-1229]

Western Blot: GNIP Antibody [NB100-1229] - Staining of Human Skeletal Muscle lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: GNIP Antibody [NB100-1229]

Immunohistochemistry-Paraffin: GNIP Antibody [NB100-1229]

Immunohistochemistry-Paraffin: GNIP Antibody [NB100-1229] - (3.8ug/ml) staining of Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for GNIP Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 6 ug/ml

Immunohistochemistry-Paraffin

3 - 6 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

1 ug/ml
Application Notes
WB: Approx. 25-30 kDa band observed in human skeletal muscle lysate (predicted MW of 25 kDa band according to NP_203128). IHC-P: Human skeletal muscle shows pixulate staining in the fibres.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GNIP

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1, a B-box type 2, and a coiled-coil region. The protein localizes to both the nucleus and the cytoplasm, and may represent a participant in the initiation of glycogen synthesis. Multiple transcript variants have been found for this gene, and some of them encode the same isoform.

Alternate Names

Glycogenin-interacting protein, GNIPRING finger protein 90, RNF90tripartite motif protein TRIM7, tripartite motif containing 7, tripartite motif-containing 7, tripartite motif-containing protein 7

Entrez Gene IDs

81786 (Human)

Gene Symbol

TRIM7

UniProt

NP_203128. (Human)

Additional GNIP Products

Product Documents for GNIP Antibody

Certificate of Analysis

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Product Specific Notices for GNIP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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