GO Protein alpha Antibody

Novus Biologicals | Catalog # NBP2-16703

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GO Protein alpha Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GO Protein alpha. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GO Protein alpha Antibody

Immunohistochemistry-Frozen: GO Protein alpha Antibody [NBP2-16703]

Immunohistochemistry-Frozen: GO Protein alpha Antibody [NBP2-16703]

Immunohistochemistry-Frozen: GO Protein alpha Antibody [NBP2-16703] - Frozen-sectioned mouse cerebellum. Green: GNAO1 stained by GNAO1 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: GO Protein alpha Antibody [NBP2-16703]

Immunohistochemistry-Paraffin: GO Protein alpha Antibody [NBP2-16703]

Immunohistochemistry-Paraffin: GO Protein alpha Antibody [NBP2-16703] - Colon carcinoma. GNAO1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: GO Protein alpha Antibody [NBP2-16703]

Immunohistochemistry-Paraffin: GO Protein alpha Antibody [NBP2-16703]

Immunohistochemistry-Paraffin: GO Protein alpha Antibody [NBP2-16703] - Mouse pancreas. GNAO1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: GO Protein alpha Antibody [NBP2-16703]

Immunohistochemistry-Paraffin: GO Protein alpha Antibody [NBP2-16703]

Immunohistochemistry-Paraffin: GO Protein alpha Antibody [NBP2-16703] - Rat pancreas. GNAO1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: GO Protein alpha Antibody [NBP2-16703]

Immunoprecipitation: GO Protein alpha Antibody [NBP2-16703]

Immunoprecipitation: GO Protein alpha Antibody [NBP2-16703] - GNAO1 protein from HeLa whole cell extracts using 5 ug of GNAO1 antibody (NBP2-16703). Western blot analysis was performed using GNAO1 antibody (NBP2-16703). EasyBlot anti-Rabbit IgG was used as a secondary reagent.
GO Protein alpha Antibody

Immunohistochemistry-Paraffin: GO Protein alpha Antibody [NBP2-16703] -

Immunohistochemistry-Paraffin: GO Protein alpha Antibody [NBP2-16703] - Analysis of paraffin-embedded zebrafish tissue, using GNAO1 antibody at 1:300 dilution.
GO Protein alpha Antibody

Western Blot: GO Protein alpha Antibody [NBP2-16703] -

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody (NBP2-16703) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GO Protein alpha Antibody

Immunocytochemistry/ Immunofluorescence: GO Protein alpha Antibody [NBP2-16703] -

GNAO1 antibody detects GNAO1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GNAO1 stained by GNAO1 antibody (NBP2-16703) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.
Blue: Fluoroshield with DAPI.
GO Protein alpha Antibody

Western Blot: GO Protein alpha Antibody [NBP2-16703] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody (NBP2-16703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GO Protein alpha Antibody

Western Blot: GO Protein alpha Antibody [NBP2-16703] -

Sample (30 ug of whole cell lysate)
A: adult zebrafish
10% SDS PAGE
NBP2-16703 diluted at 1:5000
GO Protein alpha Antibody

Western Blot: GO Protein alpha Antibody [NBP2-16703] -

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody (NBP2-16703) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for GO Protein alpha Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GO Protein alpha

Alternate Names

DKFZp686O0962, G-ALPHA-o, GNAO, guanine nucleotide binding protein (G protein), alpha activating activitypolypeptide O, guanine nucleotide binding protein, alpha activating polypeptide O, guanine nucleotide-binding protein G(o) subunit alpha

Gene Symbol

GNAO1

UniProt

P09471 (Human)

Additional GO Protein alpha Products

Product Documents for GO Protein alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GO Protein alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for GO Protein alpha Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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