Human/Mouse/Rat Syntaxin 1A Antibody

Detection of Human, Mouse, and Rat Syntaxin 1A by Western Blot.

Western blot shows lysates of human brain (cerebellum) tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Syntaxin 1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7237) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Syntaxin 1A at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Syntaxin 1A in SH-SY5Y Human Cell Line.

SH-SY5Y human neuroblastoma cells were cultured overnight in the presence of 1 mM Retinoic Acid (Catalog # 0695/50) prior to immersion fixation. Syntaxin 1A was detected using a Goat Anti-Human/Mouse/Rat Syntaxin 1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7237). The cells were stained with the NorthernLights 557-conjugated Donkey Anti-Goat IgG Affinity-purified Secondary Antibody (red; Catalog # NL001). Actin filaments were stained with FITC-conjugated Phalloidin (green) and the cell nuclei were counter-stained with DAPI (blue). Syntaxin 1A immunoreactivity was localized to synaptic vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Mouse Syntaxin 1A by Western Blot

Effect of chronic administration of EVOO‐rich diet on hippocampal pre‐ and postsynaptic protein expression in hTau mice. (a) Representative Western blot analyses for SNAP25, syntaxin 1A, synaptobrevin, complexin 1/2, and synaptophysin (SYP) in hippocampus homogenates from CTR and EVOO mice. (b) Densitometric analyses of the immunoreactivities to the antibodies shown in panel A (**p < .01; n = 6 per group). (c) Representative Western blot analyses for the AMPAR subunit glutamate receptor 1 (GluR1), AMPAR subunit glutamate receptor 2 (GluR2), and their phosphorylated forms, pGluR1 (S831), pGluR1 (S845), and pGluR2 (S880), in hippocampus homogenates from CTR and EVOO mice. (d) Densitometric analyses of the immunoreactivities to the antibodies shown in panel A (n = 6 per group). Values are mean ± SEM Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31762202), licensed under a CC-BY license. Not internally tested by R&D Systems.