GOLGA3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-91952

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GOLGA3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: STRGTYGILSKTVGTQDTPYMVNGQEIPADTLGQFPSIKDVLQAAAAEHQDQGQEVNGEVRSRRDSICSSVSLESSAAETQEEMLQVLKEKMRLEG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GOLGA3 Antibody - BSA Free

Western Blot: GOLGA3 Antibody [NBP1-91952]

Western Blot: GOLGA3 Antibody [NBP1-91952]

Western Blot: GOLGA3 Antibody [NBP1-91952] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: GOLGA3 Antibody [NBP1-91952]

Immunocytochemistry/ Immunofluorescence: GOLGA3 Antibody [NBP1-91952]

Immunocytochemistry/Immunofluorescence: GOLGA3 Antibody [NBP1-91952] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952]

Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952]

Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952]

Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952]

Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952] - Staining of human colon shows strong cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952]

Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952]

Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952] - Staining of human colon, prostate, testis and tonsil using Anti-GOLGA3 antibody NBP1-91952 (A) shows similar protein distribution across tissues to independent antibody NBP1-91953 (B).
Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952]

Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952]

Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952]

Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952]

Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952] - Staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts.
GOLGA3 Antibody - BSA Free Western Blot: GOLGA3 Antibody - BSA Free [NBP1-91952]

Western Blot: GOLGA3 Antibody - BSA Free [NBP1-91952]

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Lane 4: Human plasma (IgG/HSA depleted)
Lane 5: Human liver tissue
Lane 6: Human tonsil tissue

Applications for GOLGA3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GOLGA3

GOLGA3 is a member of the golgin family of proteins which are localized to the Golgi. It has been postulated to play a role in nuclear transport and Golgi apparatus localization.

Alternate Names

GCP170Golgi peripheral membrane protein, golgi autoantigen, golgin subfamily a, 3, Golgi complex-associated protein of 170 kD, Golgi complex-associated protein of 170 kDa, Golgi membrane associated protein, golgin A3, Golgin subfamily A member 3, golgin-160, golgin-165, male enhanced antigen-2, MEA-2, SY2/SY10 protein

Gene Symbol

GOLGA3

Additional GOLGA3 Products

Product Documents for GOLGA3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GOLGA3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GOLGA3 Antibody - BSA Free

Customer Reviews for GOLGA3 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review GOLGA3 Antibody - BSA Free and earn rewards!

Have you used GOLGA3 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies