GOLGB1/Giantin Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-22321

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GOLGB1/Giantin Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 425 to 475 of human Golgin B1 using the numbering given in entry NP_004478.3 (GeneID 2804).

Marker

Golgi Apparatus Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GOLGB1/Giantin Antibody - BSA Free

Western Blot: GOLGB1/Giantin Antibody [NBP2-22321]

Western Blot: GOLGB1/Giantin Antibody [NBP2-22321]

Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - HeLa cell lysate sample treated with DMSO was run on 8% SDS-PAGE. Both giantin monomer and dimer are indicated. Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321]

Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321]

Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - PC-3 human prostate cancer cell line. Image was captured using EVOS M5000 microscope. Red - giantin; blue - nucleus, DAPI; bar 40 micron. ICC/IF image submitted by a verified customer review.
Western Blot: GOLGB1/Giantin Antibody [NBP2-22321]

Western Blot: GOLGB1/Giantin Antibody [NBP2-22321]

Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22321 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321]

Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321]

GOLGB1-Giantin-Antibody-Immunofluorescence-NBP2-22321-img0005.jpg
Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321]

Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321]

GOLGB1-Giantin-Antibody-Immunofluorescence-NBP2-22321-img0009.jpg
Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321]

Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321]

GOLGB1-Giantin-Antibody-Immunofluorescence-NBP2-22321-img0008.jpg
Immunoprecipitation: GOLGB1/Giantin Antibody [NBP2-22321]

Immunoprecipitation: GOLGB1/Giantin Antibody [NBP2-22321]

Immunoprecipitation: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22321 used for IP at 6 ug/mg lysate. GOLGB1 was also immunoprecipitated by rabbit anti-GOLGB1 antibodies NBP2-22322 and NBP2-22323. For blotting immunoprecipitated GOLGB1, NBP2-22321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
GOLGB1/Giantin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody - BSA Free [NBP2-22321] -

Effect of Golgi disrupting treatments on the Golgi apparatus of MDA-MB-231 cells.Cells were left untreated (A; Control), or transfected to transiently express the HA-epitope-tagged ARF1 constitutively-activated mutant for 16 h (B; HA-ARF1-Q71L), or treated for 60 min either with 5 μg/ml Brefeldin A (C; BFA) or 10 μM Golgicide A (D; GCA). Cells were fixed, permeabilized, and immunolabeled with mouse monoclonal antibody to GM130, rabbit polyclonal antibody to Giantin, and sheep antibody to TGN46. Secondary antibodies were Alexa-594-conjugated donkey anti-mouse IgG (red channel), Alexa-488-conjugated donkey anti-rabbit IgG (green channel), and Alexa-647-conjugated donkey anti-sheep IgG (blue channel). Nuclei were stained with DAPI (gray channel). Stained cells were examined by fluorescence microscopy. Merging red, green, blue, and grey channels generated the fourth image on each row; yellow indicates overlapping localization of the red and green channels, cyan indicates overlapping localization of the green and blue channels, magenta indicates overlapping localization of the red and blue channels, and white indicates overlapping localization of all three channels. Bar, 10 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29614107), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GOLGB1/Giantin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody - BSA Free [NBP2-22321] -

Effect of Actinomycin D and Vinblastine on the Golgi apparatus of MDA-MB-231 cells.Cells were left untreated (A; Control), or treated for 60 min either with 10 ng/ml Actinomycin D (B; ActD) or 25 nM Vinblastine (C; VLB). Cells were fixed, permeabilized, and immunolabeled with mouse monoclonal antibody to GM130, rabbit polyclonal antibody to Giantin, and sheep antibody to TGN46. Secondary antibodies were Alexa-594-conjugated donkey anti-mouse IgG (red channel), Alexa-488-conjugated donkey anti-rabbit IgG (green channel), and Alexa-647-conjugated donkey anti-sheep IgG (blue channel). Nuclei were stained with DAPI (gray channel). Stained cells were examined by fluorescence microscopy. Merging red, green, blue, and grey channels generated the fourth image on each row; yellow indicates overlapping localization of the red and green channels, cyan indicates overlapping localization of the green and blue channels, magenta indicates overlapping localization of the red and blue channels, and white indicates overlapping localization of all three channels. Bar, 10 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29614107), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for GOLGB1/Giantin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Validated from a verified customer review.

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000

Reviewed Applications

Read 2 reviews rated 5 using NBP2-22321 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GOLGB1/Giantin

The giantin (GOLGB1) gene codes for a golgin subfamily B member 1 protein that exists in isoform 1 at a length of 3,259 amino acids and a mass of 376 kDa or isoform 2 at 3,269 amino acids long and 377 kDA. GOLGB1 is thought to participate in the development of intercisternal cross-bridges of the Golgi complex. Researchers have investigated its role in hypertension, myopathy, hematopoiesis, encephalitis, and arthritis. GOLGB1 is known to interact with other genes such as PFN2, CAV2, USO1, SLC2A3, and GORASP2.

Long Name

Golgin B1

Alternate Names

GCP372, Giantin, GOLIM1, Macrogolgin

Gene Symbol

GOLGB1

UniProt

NP_004478.3 (Human)

Additional GOLGB1/Giantin Products

Product Documents for GOLGB1/Giantin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GOLGB1/Giantin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Inflammatory Mediators

Citations for GOLGB1/Giantin Antibody - BSA Free

Customer Reviews for GOLGB1/Giantin Antibody - BSA Free (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • GOLGB1/Giantin Antibody
    Name: Armen Petrosyan
    Application: Immunofluorescence
    Sample Tested: PC-3 cells and PC-3 human prostate cancer cell line
    Species: Human and PC-3 cells
    Verified Customer | Posted 08/19/2020
    Image was captured using EVOS M5000 microscope. Red - giantin; blue - nucleus, DAPI; bar 40 mcm.
    GOLGB1/Giantin Antibody - BSA Free NBP2-22321
  • GOLGB1/Giantin Antibody
    Name: Armen Petrosyan
    Application: Western Blot
    Sample Tested: HeLa Cell Lysate
    Species: Human
    Verified Customer | Posted 11/20/2018
    HeLa cell lysate sample treated with DMSO was run on 8% SDS-PAGE. Sample was prepared in Laemmli buffer containing 10% beta-mercaptoethanol and heated for 10 min at 100 C. Both giantin monomer and dimer are indicated.
    GOLGB1/Giantin Antibody - BSA Free NBP2-22321

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