Golgi Complex Antibody (AE-6)

Novus Biologicals | Catalog # NBP2-45214

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse (Negative), Rat (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # AE-6
Loading...

Product Specifications

Immunogen

SU-DHL-1 large cell lymphoma cells

Reactivity Notes

Does not react with Mouse or Rat.

Localization

Golgi complex in cytoplasm

Marker

Marker for Human Cells

Specificity

This monoclonal antibody recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP3-11427)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Golgi Complex Antibody (AE-6)

Immunohistochemistry-Paraffin: Golgi Complex Antibody (AE-6) [NBP2-45214]

Immunohistochemistry-Paraffin: Golgi Complex Antibody (AE-6) [NBP2-45214]

Immunohistochemistry-Paraffin: Golgi Complex Antibody (AE-6) [NBP2-45214] - Human Pancreas stained with Golgi Monoclonal Antibody (AE-6).

Applications for Golgi Complex Antibody (AE-6)

Application
Recommended Usage

Flow Cytometry

0.5-1.0 ug/million cells

Immunocytochemistry

0.5 - 1.0 ug/ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunocytochemistry (Acetone or paraformaldehyde fixed): 1-2ug/ml for 30 min.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Golgi Complex

Alternate Names

AI593353;AW537898;CFR;CFR1;CFR-1;ESL1;ESL-1;GLG1;MG160;MG-160;Selel

Additional Golgi Complex Products

Product Documents for Golgi Complex Antibody (AE-6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Golgi Complex Antibody (AE-6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Golgi Complex Antibody (AE-6)

There are currently no reviews for this product. Be the first to review Golgi Complex Antibody (AE-6) and earn rewards!

Have you used Golgi Complex Antibody (AE-6)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies
Loading...