GOLM1 Antibody (OTI4B12)

Novus Biologicals | Catalog # NBP2-01639

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4B12
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Product Specifications

Immunogen

Full length human recombinant protein of human GOLM1(NP_808800) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

45.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GOLM1 Antibody (OTI4B12)

Western Blot: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Western Blot: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Western Blot: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GOLM1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Immunocytochemistry/Immunofluorescence: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GOLM1.
Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Staining of paraffin-embedded Human prostate tissue using anti-GOLM1 mouse monoclonal antibody.
Flow Cytometry: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Flow Cytometry: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Flow Cytometry: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Analysis of Hela cells, using anti-GOLM1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Western Blot: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Western Blot: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Western Blot: GOLM1 Antibody (OTI4B12) [NBP2-01639] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GOLM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GOLM1.
Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GOLM1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GOLM1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Staining of paraffin-embedded Human colon tissue using anti-GOLM1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Staining of paraffin-embedded Human endometrium tissue using anti-GOLM1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639]

Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Staining of paraffin-embedded Human Kidney tissue using anti-GOLM1 mouse monoclonal antibody.

Applications for GOLM1 Antibody (OTI4B12)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200-500

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GOLM1

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq].

Alternate Names

bA379P1.3, FLJ23608, golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2C9orf155, golgi protein, 73-kD, GOLPH2 FLJ22634, GP73, GP73 chromosome 9 open reading frame 155, PSEC0257

Entrez Gene IDs

51280 (Human)

Gene Symbol

GOLM1

Additional GOLM1 Products

Product Documents for GOLM1 Antibody (OTI4B12)

Certificate of Analysis

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Product Specific Notices for GOLM1 Antibody (OTI4B12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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