GOLM1 Antibody (OTI4B12) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71916

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4B12

Format

Azide and BSA Free
View all GOLM1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GOLM1(NP_808800) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

45.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GOLM1 Antibody (OTI4B12) - Azide and BSA Free

Western Blot: GOLM1 Antibody (OTI4B12)Azide and BSA Free [NBP2-71916]

Western Blot: GOLM1 Antibody (OTI4B12)Azide and BSA Free [NBP2-71916]

Western Blot: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GOLM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GOLM1.
Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916]

Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916]

Immunocytochemistry/Immunofluorescence: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GOLM1.
Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916]

Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916]

Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - Staining of paraffin-embedded Human Kidney tissue using anti-GOLM1 mouse monoclonal antibody.
Flow Cytometry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916]

Flow Cytometry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916]

Flow Cytometry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - Analysis of Hela cells, using anti-GOLM1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Western Blot: GOLM1 Antibody (OTI4B12)Azide and BSA Free [NBP2-71916]

Western Blot: GOLM1 Antibody (OTI4B12)Azide and BSA Free [NBP2-71916]

Western Blot: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GOLM1 monoclonal antibody.
Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916]

Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916]

Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GOLM1 mouse monoclonal antibody.
Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916]

Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916]

Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GOLM1 mouse monoclonal antibody.
Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916]

Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916]

Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - Staining of paraffin-embedded Human colon tissue using anti-GOLM1 mouse monoclonal antibody.
Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916]

Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916]

Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - Staining of paraffin-embedded Human endometrium tissue using anti-GOLM1 mouse monoclonal antibody.
Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916]

Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916]

Immunohistochemistry: GOLM1 Antibody (OTI4B12) - Azide and BSA Free [NBP2-71916] - Staining of paraffin-embedded Human prostate tissue using anti-GOLM1 mouse monoclonal antibody.

Applications for GOLM1 Antibody (OTI4B12) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200-500

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GOLM1

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by GOLM1 is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of GOLM1 has been observed to be upregulated in response to viral infection. Two alternatively spliced transcript variants encoding the same protein have been described for GOLM1.

Alternate Names

bA379P1.3, FLJ23608, golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2C9orf155, golgi protein, 73-kD, GOLPH2 FLJ22634, GP73, GP73 chromosome 9 open reading frame 155, PSEC0257

Gene Symbol

GOLM1

Additional GOLM1 Products

Product Documents for GOLM1 Antibody (OTI4B12) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GOLM1 Antibody (OTI4B12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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