GOLM1 Antibody

Novus Biologicals | Catalog # NBP1-50627

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GOLM1 Primary Antibodies »

Product Specifications

Immunogen

A genomic peptide made to an internal region of the human GOLM1 protein (within residues 250-400). [Swiss-Prot Q8NBJ4]

Localization

Golgi apparatus - cis-Golgi network membrane

Marker

Golgi Apparatus Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GOLM1 Antibody

Knockout Validated: GOLM1 Antibody [NBP1-50627]

Western Blot: GOLM1 Antibody [NBP1-50627]

Western Blot: GOLM1 Antibody [NBP1-50627] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and GOLM1 knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:5,000 of Rabbit Anti-Human GOLM1 polyclonal Antibody (Catalog # NBP1-50627) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for GOLM1 at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.
Western Blot: GOLM1 Antibody [NBP1-50627]

Western Blot: GOLM1 Antibody [NBP1-50627]

Western Blot: GOLM1 Antibody [NBP1-50627] - Western Blot Image of GOLM1. Whole cell protein from HeLa (lane 1), 3T3 (lane 2), MEF (lane 3), Raw264.7 (lane 4), L929 (lane 5), Neuro2A (lane 6) and PC12 (lane 7) were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 mg/ml anti-GOLM1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody [NBP1-50627]

Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody [NBP1-50627]

Immunocytochemistry/Immunofluorescence: GOLM1 Antibody [NBP1-50627] - Confocal immunofluorescent analysis of HeLa cells using GOLM1 antibody (NBP1-50627, 1:25). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody [NBP1-50627]

Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody [NBP1-50627]

Immunocytochemistry/Immunofluorescence: GOLM1 Antibody [NBP1-50627] - ICC staining of GOLM1 in HEK293T cells with FITC (green). Nuclei (blue) were counterstained with Dapi.
Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody [NBP1-50627]

Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody [NBP1-50627]

Immunocytochemistry/Immunofluorescence: GOLM1 Antibody [NBP1-50627] - GOLM1 antibody was tested in HEK-293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Applications for GOLM1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Western Blot

1:5000

Reviewed Applications

Read 3 reviews rated 4.7 using NBP1-50627 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

0.61 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GOLM1

In liver, prostate and lung, both viral and non-viral related disease is correlated to the upregulation of GOLPH2, also known as GOLM1, GP73 or Golgi phosphoprotein 2. While it remains unclear how viral insertions are manifested into the Golgi apparatus and subsequent shepherding of proteins, GOLPH2 seems to be affected by the insertions in such a way that it can be attributed to disease phenotypes. Furthermore, because GOLPH2 is so uniquely regulated in cells, it can be successfully used as a clinically relevant molecular biomarker for cancer. As it is secreted and excreted, GOLPH2 is a useful biomarker because of relative ease of sampling in urine and direct correlation to urine concentrations and disease.

Alternate Names

bA379P1.3, FLJ23608, golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2C9orf155, golgi protein, 73-kD, GOLPH2 FLJ22634, GP73, GP73 chromosome 9 open reading frame 155, PSEC0257

Entrez Gene IDs

51280 (Human)

Gene Symbol

GOLM1

UniProt

Q8NBJ4 (Human)

Additional GOLM1 Products

Product Documents for GOLM1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GOLM1 Antibody

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for GOLM1 Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • GOLM1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: human liver and primary hepatocytes
    Species: Human
    Verified Customer | Posted 12/15/2017
    human liver and primary hepatocytes
    human liver and primary hepatocytes
    GOLM1 Antibody NBP1-50627
  • Name: Bryan Tinsley
    Application: Immunocytochemistry
    Sample Tested:
    Species: Human
    Verified Customer | Posted 06/12/2014
    Confocal immunofluorescent analysis of HeLa cells using GOLM1 antibody (NBP1-50627, 1:25).
    GOLM1 Antibody NBP1-50627
  • Name: Bryan Tinsley
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 06/12/2014
    Western blot analysis of extracts from LNCaP cells using GOLM1 antibody (NBP1-50627, 1:5000).
    GOLM1 Antibody NBP1-50627

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Protocols

View specific protocols for GOLM1 Antibody (NBP1-50627):

GOLM1 Antibody:
Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes.
2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.

GOLM1 Antibody:
Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute the primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.

*Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

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FAQs

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