gp96/HSP90B1/GRP94 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32118

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all gp96/HSP90B1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human gp96/HSP90B1/GRP94. The exact sequence is proprietary.

Localization

Cytoplasm, Melanosome

Marker

ER Stress Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for gp96/HSP90B1/GRP94 Antibody - BSA Free

Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118]

Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118]

Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody
Immunocytochemistry/ Immunofluorescence: gp96/HSP90B1/GRP94 Antibody [NBP1-32118]

Immunocytochemistry/ Immunofluorescence: gp96/HSP90B1/GRP94 Antibody [NBP1-32118]

Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - GRP94 Antibody [NBP1-32118] - Analysis of methanol-fixed HCT116, using antibody at 1:500 dilution.
Immunohistochemistry: gp96/HSP90B1/GRP94 Antibody [NBP1-32118]

Immunohistochemistry: gp96/HSP90B1/GRP94 Antibody [NBP1-32118]

Immunohistochemistry: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Mouse lung. HSP90 beta antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118]

Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118]

Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - GRP94 Antibody [NBP1-32118] - Sample(30 ug of whole cell lysate)A:293TB:A431 7. 5% SDS PAGE, antibody diluted at 1:1500.
Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118]

Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118]

Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody diluted at 1:1500. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-32118]

Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-32118]

Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - GRP94 Antibody [NBP1-32118] - Paraffin-embedded BT483 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-32118]

Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-32118]

Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Mouse skin. HSP90 beta antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
gp96/HSP90B1/GRP94 Antibody

IHC-WM-gp96/HSP90B1/GRP94 Antibody [NBP1-32118] -

IHC-WM-gp96/HSP90B1/GRP94 Antibody [NBP1-32118] -Hsp90 beta antibody detects Hsp90 beta protein on whole-mount zebrafish embryos by immunohistochemical analysis.Sample: Paraformaldehyde-fixed zebrafish embryos.Hsp90 beta antibody dilution: 1:200.
gp96/HSP90B1/GRP94 Antibody

Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] -

Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Sample (30 ug of whole cell lysate)
A: zebrafish eye
7.5% SDS PAGE
NBP1-32118 diluted at 1:10000

Applications for gp96/HSP90B1/GRP94 Antibody - BSA Free

Application
Recommended Usage

ELISA

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry Whole-Mount

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: gp96/HSP90B1

Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1

Long Name

Heat Shock Protein 90 beta 1

Alternate Names

ECGP, Endoplasmin, Grp94, HSP90B1

Gene Symbol

HSP90B1

Additional gp96/HSP90B1 Products

Product Documents for gp96/HSP90B1/GRP94 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for gp96/HSP90B1/GRP94 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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