GPER/GPR30 Antibody - BSA Free
Novus Biologicals | Catalog # NLS4272
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]](https://resources.rndsystems.com/images/products/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS4272-img0008.jpg "Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]")
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Key Product Details
Species Reactivity
Validated:
Human, Rat, Canine, Monkey
Cited:
Rat
Predicted:
Primate (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Synthetic 18 amino acid peptide from C-terminus of human GPER/GPR30.
Epitope
C-Terminus
Reactivity Notes
Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (100%), Mouse (89%), Hamster (89%), Bovine (83%).
Localization
Endoplasmic reticulum - multi-pass membrane protein.
Specificity
Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Product can be stored undiluted at 4C for up to 1 month.
Scientific Data Images for GPER/GPR30 Antibody - BSA Free
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4272] - Lung, non small cell carcinoma![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]](https://resources.rndsystems.com/images/products/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS4272-img0001.jpg "Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]")
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4272] - Analysis of anti-GPER1 / GPR30 antibody with human breast carcinoma at 10ug/ml.![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]](https://resources.rndsystems.com/images/products/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS4272-img0002.jpg "Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]")
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4272] - Analysis of anti-GPER1 / GPR30 antibody with human pancreas, carcinoma.![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]](https://resources.rndsystems.com/images/products/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS4272-img0003.jpg "Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]")
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4272] - Human Prostate, Carcinoma paraffin-embedded tissue after heat-induced antigen retrieval.![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]](https://resources.rndsystems.com/images/products/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS4272-img0004.jpg "Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]")
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4272] - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE).![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]](https://resources.rndsystems.com/images/products/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS4272-img0005.jpg "Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]")
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4272] - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]](https://resources.rndsystems.com/images/products/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS4272-img0006.jpg "Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]")
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4272] - Prostate, Adenocarcinoma![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]](https://resources.rndsystems.com/images/products/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS4272-img0007.jpg "Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]")
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4272]
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4272] - Breast, adenocarcinomaApplications for GPER/GPR30 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Frozen
1:10 - 1:500
Immunohistochemistry-Paraffin
5-10 ug/ml
Western Blot
1:100 - 1:2000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 23822769). Use in IHC reported in scientific literature (PMID: 22378360)..
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Background: GPER/GPR30
Long Name
G Protein-coupled Estrogen Receptor 1
Alternate Names
CEPR, CMKRL2, DRY12, FEG-1, GPCR-BR, GPR30, LERGU2, LyGPR
Gene Symbol
GPER1
UniProt
Additional GPER/GPR30 Products
Product Documents for GPER/GPR30 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GPER/GPR30 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for GPER/GPR30 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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