GPI-PLD/GPLD1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03599

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GPI-PLD/GPLD1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 24-160 of human GPI-PLD/GPLD1 (NP_001494.2). CGLSTHVEIGHRALEFLQLHNGRVNYRELLLEHQDAYQAGIVFPDCFYPSICKGGKFHDVSESTHWTPFLNASVHYIRENYPLPWEKDTEKLVAFLFGITSHMAADVSWHSLGLEQGFLRTMGAIDFHGSYSEAHSA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GPI-PLD/GPLD1 Antibody - BSA Free

Western Blot: GPI-PLD/GPLD1 AntibodyAzide and BSA Free [NBP3-03599]

Western Blot: GPI-PLD/GPLD1 AntibodyAzide and BSA Free [NBP3-03599]

Western Blot: GPI-PLD/GPLD1 Antibody [NBP3-03599] - Analysis of extracts of various cell lines, using GPLD1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: GPI-PLD/GPLD1 Antibody - Azide and BSA Free [NBP3-03599]

Immunohistochemistry-Paraffin: GPI-PLD/GPLD1 Antibody - Azide and BSA Free [NBP3-03599]

Immunohistochemistry-Paraffin: GPI-PLD/GPLD1 Antibody [NBP3-03599] - Rat kidney using GPI-PLD/GPLD1 Rabbit pAb (NBP3-03599) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence: GPI-PLD/GPLD1 Antibody - Azide and BSA Free [NBP3-03599]

Immunocytochemistry/ Immunofluorescence: GPI-PLD/GPLD1 Antibody - Azide and BSA Free [NBP3-03599]

Immunocytochemistry/Immunofluorescence: GPI-PLD/GPLD1 Antibody [NBP3-03599] - Analysis of A-549 cells using GPLD1 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: GPI-PLD/GPLD1 Antibody - Azide and BSA Free [NBP3-03599]

Immunohistochemistry-Paraffin: GPI-PLD/GPLD1 Antibody - Azide and BSA Free [NBP3-03599]

Immunohistochemistry-Paraffin: GPI-PLD/GPLD1 Antibody [NBP3-03599] - Rat brain using GPI-PLD/GPLD1 Rabbit pAb (NBP3-03599) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
GPI-PLD/GPLD1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GPI-PLD/GPLD1 Antibody - Azide and BSA Free [GPI-PLD/GPLD1] -

Immunocytochemistry/ Immunofluorescence: GPI-PLD/GPLD1 Antibody - Azide and BSA Free [GPI-PLD/GPLD1] - Immunofluorescence analysis of HepG2 cells using GPI-PLD/GPLD1 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for GPI-PLD/GPLD1 Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GPI-PLD/GPLD1

Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol(GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPIdegrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage inproteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane.(provided by RefSeq)

Long Name

Glycosylphosphatidylinositol Specific Phospholipase D1

Alternate Names

Glycoprotein Phospholipase D, GPIPLD, GPIPLDM, GPLD1, PIGPLD1, PLD

Gene Symbol

GPLD1

Additional GPI-PLD/GPLD1 Products

Product Documents for GPI-PLD/GPLD1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GPI-PLD/GPLD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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